{"id":"W2907852965","doi":"10.1074/jbc.ra118.006447","title":"Investigating the stability of the SecA–SecYEG complex during protein translocation across the bacterial membrane","year":2019,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Periplasmic space; ATP hydrolysis; Processivity; Biology; Transport protein; ATPase; Biophysics; Translocase; Membrane transport protein; Biochemistry; AAA proteins; Twin-arginine translocation pathway; Cell biology; Inner membrane; Escherichia coli; Membrane protein; Chromosomal translocation; Membrane; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003255593,0.0003211811,0.0002550247,0.000283889,0.0003592109,0.0006705029,0.0003381789,0.0003545898,0.0007018406],"category_scores_gemma":[0.0005267418,0.0002177291,0.0002199441,0.0003251688,0.0003777643,0.0005794943,0.0003265936,0.0007345505,0.0003002471],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004639724,"about_ca_system_score_gemma":0.0001548788,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007942902,"about_ca_topic_score_gemma":0.0004921371,"domain_scores_codex":[0.9997653,0.00004044886,0.00002151629,0.00005453599,0.00006666213,0.00005152361],"domain_scores_gemma":[0.9997048,0.0000757732,0.00007552127,0.00003091166,0.00005339848,0.00005952522],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001431569,0.00004149093,0.002096702,0.0000368679,0.00002685404,0.00008551532,0.00008782213,0.0001121571,0.9964641,0.0001208724,0.0000239248,0.0007605565],"study_design_scores_gemma":[0.00002546143,0.000415204,0.06445078,0.0000203565,0.00004891265,0.0005304617,0.000303622,0.00753279,0.9242904,0.0002123895,0.002138071,0.0000315276],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981267,0.0006095272,0.0008956056,0.00003824544,0.000007450693,0.000007202806,0.00008873221,0.000008238391,0.000218228],"genre_scores_gemma":[0.9979942,0.0003393845,0.0006976452,0.0000289752,0.000007870557,0.00001501603,0.0003441482,0.00001182162,0.0005609737],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007942902,"threshold_uncertainty_score":0.003366411,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02218433105948965,"score_gpt":0.2427473119659654,"score_spread":0.2205629809064758,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}