{"id":"W2909069340","doi":"10.3791/58709","title":"Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues","year":2019,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hotchkiss Brain Institute; Alberta Children's Hospital; University of Calgary","funders":"Canadian Institutes of Health Research; Children's Health Research Institute; University of Calgary","keywords":"Transcriptome; Biology; Cell; Computational biology; Single-cell analysis; Cell biology; RNA; Cell type; Gene expression; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001256641,0.0005498362,0.0006505111,0.0009100239,0.0007442498,0.0009762099,0.001268855,0.0008018998,0.004854325],"category_scores_gemma":[0.001084235,0.0006439713,0.0006133774,0.0007916006,0.0007891753,0.0005779405,0.000947624,0.00172454,0.004478909],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006700555,"about_ca_system_score_gemma":0.0008720883,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007409803,"about_ca_topic_score_gemma":0.003169389,"domain_scores_codex":[0.9988959,0.0001067409,0.00009872648,0.0003834856,0.000426088,0.00008903658],"domain_scores_gemma":[0.999374,0.0001881316,0.00008519417,0.0001801427,0.0001270882,0.00004544382],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002332123,0.000007249876,0.00006312961,0.00009726229,0.000004952905,0.00003400155,0.00004054291,0.0001822435,0.994355,0.001067105,0.000530656,0.003594617],"study_design_scores_gemma":[0.00002773835,0.00007738184,0.001592375,0.00004678265,0.00001638118,0.0002221233,0.00003477842,0.005299062,0.9609063,0.001228419,0.03051807,0.00003065584],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.06400383,0.001366327,0.8891609,0.0004796754,0.0004103562,0.002175254,0.02662359,0.00647334,0.00930673],"genre_scores_gemma":[0.1470148,0.002575019,0.7931089,0.0007111711,0.0001221307,0.005257061,0.03380793,0.002790496,0.01461232],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004854325,"threshold_uncertainty_score":0.01623935,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01841523030084467,"score_gpt":0.3252766353833978,"score_spread":0.3068614050825531,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}