{"id":"W2910325493","doi":"10.1107/s2059798318016595","title":"Structural studies of a surface-entropy reduction mutant of O-GlcNAcase","year":2019,"lang":"en","type":"article","venue":"Acta Crystallographica Section D Structural Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Biotechnology and Biological Sciences Research Council; Directorate for Biological Sciences; Simon Fraser University; Royal Society; Diamond Light Source","keywords":"Chemistry; Stereochemistry; Enzyme; Conformational entropy; Monomer; Active site; Mutant; Hydrolase; Crystallography; Molecule; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002431731,0.0004296381,0.0003791851,0.0001232647,0.0002607915,0.0003440301,0.0006232163,0.000705254,0.001173947],"category_scores_gemma":[0.0004304335,0.0001615403,0.0003730447,0.0002203205,0.0003685733,0.0002360357,0.0002514386,0.001396132,0.0003716309],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005129249,"about_ca_system_score_gemma":0.0003404003,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00435437,"about_ca_topic_score_gemma":0.003157861,"domain_scores_codex":[0.9998579,0.00002768786,0.00001593,0.00002288192,0.00005096138,0.0000245186],"domain_scores_gemma":[0.9997154,0.00009324077,0.00005503719,0.00001743771,0.00003659176,0.00008226326],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006976181,0.0002947081,0.0008891589,0.0001029634,0.00005100778,0.001877418,0.0002333095,0.002928099,0.9884809,0.00122513,0.0005096198,0.002710071],"study_design_scores_gemma":[0.0003105231,0.001216592,0.01405041,0.00006044232,0.0001234194,0.003373184,0.0005103913,0.03266893,0.9372506,0.0006629439,0.009683572,0.00008900184],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9967212,0.0004013359,0.001437859,0.0001711937,0.00006018945,0.00001473608,0.0003454076,0.0000407299,0.0008073648],"genre_scores_gemma":[0.9955335,0.0003606695,0.002053201,0.00006141749,0.00001141201,0.00001209817,0.0006264983,0.00005541918,0.001285792],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00435437,"threshold_uncertainty_score":0.008658051,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01696303422403345,"score_gpt":0.2966541236140799,"score_spread":0.2796910893900465,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}