{"id":"W2910936296","doi":"10.3390/microorganisms7010008","title":"Expression and Purification of Chemokine MIP-3α (CCL20) through a Calmodulin-Fusion Protein System","year":2019,"lang":"en","type":"article","venue":"Microorganisms","topic":"Antimicrobial Peptides and Activities","field":"Immunology and Microbiology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Fusion protein; Chemokine; Recombinant DNA; CCL22; Biochemistry; Thioredoxin; Maltose-binding protein; Chemistry; Biology; Molecular biology; Cell biology; Chemokine receptor; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002678549,0.0004684089,0.0004896701,0.0002742031,0.0003431979,0.0003659267,0.0005212459,0.0004598314,0.0007151074],"category_scores_gemma":[0.0002210983,0.0001897501,0.0003854294,0.0005571792,0.0001577436,0.0002635924,0.0003667822,0.0008536397,0.0009673531],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005778405,"about_ca_system_score_gemma":0.0003396554,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001273985,"about_ca_topic_score_gemma":0.001181449,"domain_scores_codex":[0.9996936,0.0000461025,0.00003713704,0.00006003595,0.0001047817,0.00005826157],"domain_scores_gemma":[0.9999006,0.00001328085,0.00002387589,0.00001211747,0.00002513225,0.00002500578],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002597874,0.00002232954,0.00005242023,0.00003847598,0.000003551989,0.00003924427,0.00001546244,0.00005556574,0.9989777,0.00009633348,0.00008117479,0.000591745],"study_design_scores_gemma":[0.00003150054,0.0001236649,0.001627703,0.00001180253,0.00001875423,0.0004045157,0.00002504671,0.003399114,0.9841138,0.00007817536,0.01015238,0.00001339758],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9134262,0.004007955,0.07371172,0.000822858,0.0002388248,0.0004942497,0.003613354,0.000520772,0.003164234],"genre_scores_gemma":[0.889715,0.00452609,0.08254038,0.0002529681,0.0001113195,0.0004192285,0.01510608,0.0001524797,0.007176429],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001273985,"threshold_uncertainty_score":0.004192531,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006947051586643605,"score_gpt":0.196665321999005,"score_spread":0.1897182704123614,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}