{"id":"W2910936296","doi":"10.3390/microorganisms7010008","title":"Expression and Purification of Chemokine MIP-3α (CCL20) through a Calmodulin-Fusion Protein System","year":2019,"lang":"en","type":"article","venue":"Microorganisms","topic":"Antimicrobial Peptides and Activities","field":"Immunology and Microbiology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"","keywords":"Fusion protein; Chemokine; Recombinant DNA; CCL22; Biochemistry; Thioredoxin; Maltose-binding protein; Chemistry; Biology; Molecular biology; Cell biology; Chemokine receptor; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001195014,0.0001831906,0.0003345448,0.00004824509,0.0001163158,0.00001551244,0.0001473183,0.000255981,0.0003594621],"category_scores_gemma":[0.00001077263,0.0001539005,0.0000588194,0.00008407882,0.0001465869,0.0001465131,0.0001270625,0.0001694012,0.0002939448],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003457457,"about_ca_system_score_gemma":0.00002823401,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001568041,"about_ca_topic_score_gemma":0.000002609997,"domain_scores_codex":[0.9990296,0.00007340912,0.0002991561,0.000331129,0.00003499888,0.0002317301],"domain_scores_gemma":[0.9993842,0.00002643461,0.0002101511,0.0003015081,0.00006380577,0.0000139203],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008915756,0.00007271013,0.0002990029,0.0002323439,0.00002503835,0.000001087611,0.0006519373,8.862077e-7,0.9967456,0.0008567968,0.0006292656,0.0003962143],"study_design_scores_gemma":[0.0009019342,0.0001265801,0.0007481737,0.0003146644,0.00001867267,0.00006382133,0.000963517,0.000002276343,0.9922162,0.00005641336,0.004414985,0.0001727421],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9967059,0.0009044631,0.0005587879,0.0001325069,0.000244378,0.0005568045,0.00004265977,0.00007378484,0.0007807362],"genre_scores_gemma":[0.9942935,0.00003272664,0.00152378,0.00004564332,0.00002724096,0.000009938397,0.0000791041,0.00002684045,0.003961208],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00452934,"threshold_uncertainty_score":0.6275882,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006947051586643605,"score_gpt":0.196665321999005,"score_spread":0.1897182704123614,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}