{"id":"W2911437499","doi":"10.1038/s42003-018-0269-2","title":"Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration","year":2019,"lang":"en","type":"article","venue":"Communications Biology","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":155,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Institute of Neurological Disorders and Stroke; Canadian Institutes of Health Research; National Institute of Diabetes and Digestive and Kidney Diseases; Intellectual and Developmental Disabilities Research Center; Alberta Innovates; National Institutes of Health; University of Alberta; National Institute of Allergy and Infectious Diseases; Fondation Brain Canada; Natural Sciences and Engineering Research Council of Canada; Harvard Digestive Diseases Center, Harvard University; Burroughs Wellcome Fund","keywords":"Förster resonance energy transfer; Green fluorescent protein; Fluorescence; Cytosol; Biophysics; Intracellular; Fluorescent protein; Live cell imaging; Potassium; Chemistry; Cell biology; Biochemistry; Efflux; Fluorescence-lifetime imaging microscopy; Biology; Cell; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003110755,0.0004968274,0.0002936185,0.0003409068,0.0002115965,0.0004034446,0.0005899005,0.00071419,0.0008512889],"category_scores_gemma":[0.0003646252,0.0002465331,0.0003030159,0.0001930958,0.0003762069,0.0004436452,0.0006380095,0.0009584611,0.000550592],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009144185,"about_ca_system_score_gemma":0.0002796001,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003872136,"about_ca_topic_score_gemma":0.0005839301,"domain_scores_codex":[0.999743,0.00004391921,0.00001691519,0.0000636828,0.00009061032,0.00004182647],"domain_scores_gemma":[0.99979,0.00003669032,0.00007378514,0.00002679581,0.00003258111,0.0000402122],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001869152,0.000005192448,0.00006088947,0.00003073124,0.000002803991,0.00002099197,0.000006551687,0.0001623871,0.9980995,0.0003083136,0.00008067591,0.001203243],"study_design_scores_gemma":[0.000006030228,0.00003407438,0.0003062553,0.000004680527,0.000006424417,0.0001677902,0.000005739665,0.001680845,0.9948062,0.00008412798,0.002889088,0.000008706622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6455194,0.004335618,0.3398702,0.001075661,0.0002644474,0.0002211362,0.001312858,0.002110033,0.005290646],"genre_scores_gemma":[0.7675987,0.002539833,0.2227957,0.0002537405,0.00003285821,0.00028726,0.001166784,0.0002315168,0.005093568],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009144185,"threshold_uncertainty_score":0.006634653,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01317587119857261,"score_gpt":0.2709489966443176,"score_spread":0.257773125445745,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}