{"id":"W2911437499","doi":"10.1038/s42003-018-0269-2","title":"Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration","year":2019,"lang":"en","type":"article","venue":"Communications Biology","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":155,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Institute of Neurological Disorders and Stroke; Canadian Institutes of Health Research; National Institute of Diabetes and Digestive and Kidney Diseases; Intellectual and Developmental Disabilities Research Center; Alberta Innovates; National Institutes of Health; University of Alberta; National Institute of Allergy and Infectious Diseases; Fondation Brain Canada; Natural Sciences and Engineering Research Council of Canada; Harvard Digestive Diseases Center, Harvard University; Burroughs Wellcome Fund","keywords":"Förster resonance energy transfer; Green fluorescent protein; Fluorescence; Cytosol; Biophysics; Intracellular; Fluorescent protein; Live cell imaging; Potassium; Chemistry; Cell biology; Biochemistry; Efflux; Fluorescence-lifetime imaging microscopy; Biology; Cell; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002129194,0.0001138482,0.0001103443,0.00006434148,0.000146337,0.00001885008,0.0003699646,0.0001576354,0.00005076205],"category_scores_gemma":[0.00007901432,0.0001127603,0.00007482713,0.0001042953,0.0001554309,0.00000524638,0.000152702,0.00008431922,0.00004016186],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002800445,"about_ca_system_score_gemma":0.0000673107,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003373723,"about_ca_topic_score_gemma":0.000003804384,"domain_scores_codex":[0.9991102,0.0001321249,0.0002486014,0.0002657636,0.00004696674,0.0001963718],"domain_scores_gemma":[0.9987885,0.00003744264,0.0001158527,0.0008676352,0.000132391,0.00005817326],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00007613413,0.00009031628,0.015618,0.00001038027,0.00003018179,4.467768e-8,0.00004477657,0.0001321153,0.950279,0.011517,0.001444124,0.02075791],"study_design_scores_gemma":[0.001975538,0.0004704758,0.005228641,0.00001683772,0.00004016006,0.00001176795,0.0001888497,0.04939546,0.4058715,0.001254786,0.5350855,0.0004605514],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8385951,0.004376457,0.1429129,0.004884998,0.001922001,0.002036517,0.00008530309,0.00009738647,0.005089329],"genre_scores_gemma":[0.9931521,0.0003713944,0.003208596,0.0003469787,0.000201993,0.00009351347,0.002320468,0.00001662543,0.0002883223],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.5444075,"threshold_uncertainty_score":0.4598233,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01317587119857261,"score_gpt":0.2709489966443176,"score_spread":0.257773125445745,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}