{"id":"W2911943528","doi":"10.1111/pbi.13092","title":"Activity‐based proteomics reveals nine target proteases for the recombinant protein‐stabilizing inhibitor <i>Sl</i><scp>CYS</scp>8 in <i>Nicotiana benthamiana</i>","year":2019,"lang":"en","type":"article","venue":"Plant Biotechnology Journal","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Fonds de recherche du Québec – Nature et technologies; Natural Sciences and Engineering Research Council of Canada; Horizon 2020 Framework Programme; H2020 European Research Council; Deutsche Forschungsgemeinschaft","keywords":"Proteases; Cystatin; Nicotiana benthamiana; Biology; Biochemistry; Proteolysis; Cysteine protease; Recombinant DNA; Protease; Cathepsin; Serine protease; Lipid-anchored protein; Enzyme; Cystatin C","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001052985,0.0003258512,0.0002141504,0.0001276259,0.0001000808,0.0002664263,0.0001806171,0.0002089791,0.0004125353],"category_scores_gemma":[0.00008539535,0.0001126733,0.0002425585,0.0001261892,0.0001347904,0.0001647662,0.0001059087,0.0003532513,0.0002362887],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000392414,"about_ca_system_score_gemma":0.0001736792,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001202311,"about_ca_topic_score_gemma":0.002097751,"domain_scores_codex":[0.9999131,0.000009009057,0.00000957356,0.00002803297,0.00002652271,0.00001371437],"domain_scores_gemma":[0.9999151,0.00001375049,0.00002899168,0.000009342688,0.00001371255,0.000019113],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001740115,0.000002151024,0.0001188052,0.000007250192,0.000001783853,0.000008834938,0.000003428388,0.000007962991,0.9996049,0.000006803426,0.000004811858,0.0002158977],"study_design_scores_gemma":[0.000003820012,0.0000510468,0.008346853,0.000002127469,0.00001553824,0.0001872427,0.00001552775,0.0005917163,0.9898155,0.00001471869,0.0009536365,0.000002415967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993828,0.0008481634,0.004104279,0.00006981318,0.000006286333,0.00001528081,0.0005269552,0.00008658603,0.000514659],"genre_scores_gemma":[0.9909782,0.0005785913,0.004297331,0.00006848356,0.000005186222,0.00002070167,0.002143982,0.00004826934,0.001859248],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001202311,"threshold_uncertainty_score":0.002847135,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008676090553829187,"score_gpt":0.2132555235961155,"score_spread":0.2045794330422863,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}