{"id":"W2914552035","doi":"10.1038/s41596-018-0122-6","title":"Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans","year":2019,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Antifungal resistance and susceptibility","field":"Medicine","cited_by":37,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Banting Research Foundation; Natural Sciences and Engineering Research Council of Canada; Burroughs Wellcome Fund","keywords":"CRISPR; Candida albicans; Biology; Cas9; Genome editing; Computational biology; Gene; Plasmid; Genetics; Genome; Model organism","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001104937,0.0006124685,0.0004664978,0.0006658825,0.0007267056,0.001357504,0.001437347,0.0005613549,0.001421222],"category_scores_gemma":[0.0009547971,0.0006071204,0.0005023609,0.0003593207,0.0006979044,0.0003896703,0.0006456951,0.001495791,0.0005805594],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001414251,"about_ca_system_score_gemma":0.001599718,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002045247,"about_ca_topic_score_gemma":0.00408106,"domain_scores_codex":[0.9988552,0.0001343876,0.0001595145,0.0002590605,0.0004443551,0.0001473736],"domain_scores_gemma":[0.9993075,0.0001825456,0.0001771993,0.0001329114,0.00009167553,0.000108181],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001916472,0.0001179599,0.000491159,0.00007717552,0.0000153248,0.00009101455,0.00005571041,0.003599742,0.9863365,0.002106239,0.0003117547,0.006605884],"study_design_scores_gemma":[0.00003462717,0.0001490877,0.0009353545,0.00001223547,0.0000207162,0.0000811805,0.00003513914,0.008752995,0.9843981,0.0002158445,0.00534269,0.0000220161],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7381201,0.001053507,0.242775,0.0007864402,0.0002778369,0.001836971,0.004454209,0.003718285,0.006977521],"genre_scores_gemma":[0.8758724,0.0006702151,0.1134746,0.0002105951,0.00001829789,0.0008024584,0.00289754,0.0005091082,0.0055448],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002045247,"threshold_uncertainty_score":0.01026118,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01592088385695474,"score_gpt":0.332016212895354,"score_spread":0.3160953290383993,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}