{"id":"W2915616991","doi":"10.1016/j.nbt.2019.02.006","title":"Pichia pastoris as a host for production and isolation of mutagenic AID/APOBEC enzymes involved in cancer and immunity","year":2019,"lang":"en","type":"article","venue":"New Biotechnology","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"Memorial University of Newfoundland","funders":"Faculty of Medicine, Memorial University of Newfoundland; Canadian Institutes of Health Research; International Development Research Centre; Beatrice Hunter Cancer Research Institute","keywords":"Biology; Pichia pastoris; Enzyme; Fusion protein; Biochemistry; Yeast; Mutagenesis; Gene; Molecular biology; Recombinant DNA; Mutation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001157387,0.00008255116,0.0002232124,0.0002083891,0.00003004466,0.000003012156,0.00004639734,0.0002207437,0.00003713737],"category_scores_gemma":[0.0001328846,0.00006885177,0.00002326561,0.0001773253,0.0001495321,0.00004279894,0.00006341671,0.0002223752,0.000003455376],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003394125,"about_ca_system_score_gemma":0.0001080365,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004747103,"about_ca_topic_score_gemma":0.001966872,"domain_scores_codex":[0.9994103,0.00001570652,0.0001391954,0.0002013845,0.00007884131,0.0001545247],"domain_scores_gemma":[0.9996667,0.00004342373,0.00005268072,0.0001514235,0.00004637414,0.00003943426],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009895422,0.00006609949,0.2138639,0.0002999654,0.00004792055,0.000001404943,0.0003770701,7.251947e-7,0.7592993,0.002454497,0.0003973505,0.02220223],"study_design_scores_gemma":[0.002942273,0.002399518,0.201736,0.0002752695,0.00004421433,0.00005502972,0.0008148895,0.0005119643,0.7530611,0.001422312,0.03654066,0.0001967065],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.977257,0.003915383,0.00002989198,0.01805675,0.0001045042,0.0005603922,0.000007454261,0.00002176406,0.00004681548],"genre_scores_gemma":[0.9943941,0.00249018,0.0008190515,0.00007189842,0.00006806974,0.00001964743,0.000009286972,0.000008344643,0.002119436],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03614331,"threshold_uncertainty_score":0.7176231,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02298611242983465,"score_gpt":0.3134142960165548,"score_spread":0.2904281835867202,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}