{"id":"W2937318362","doi":"10.1007/978-1-4939-9121-1_14","title":"Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay","year":2019,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Ottawa","funders":"Canadian Institutes of Health Research","keywords":"G protein-coupled receptor; Arrestin; Functional selectivity; G protein; Signal transduction; Receptor; Computational biology; Biology; Cell biology; Intracellular; HEK 293 cells; Chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005036209,0.0009938186,0.0007698364,0.001167076,0.001179799,0.001051754,0.0008227066,0.001046043,0.006036139],"category_scores_gemma":[0.0005890052,0.0004343702,0.0007161256,0.0008521545,0.0005546054,0.0008557221,0.0008946566,0.002232771,0.002212607],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009760727,"about_ca_system_score_gemma":0.0005325363,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001218839,"about_ca_topic_score_gemma":0.003176435,"domain_scores_codex":[0.9991578,0.0001360553,0.00005548054,0.0002146167,0.0002701299,0.0001659399],"domain_scores_gemma":[0.9994408,0.0001497982,0.0001125267,0.00005978186,0.00006849992,0.0001686184],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001212408,0.00005937892,0.0003318403,0.0000724339,0.00002375257,0.0000726557,0.00006886105,0.00008207453,0.9970536,0.0005141378,0.0003765337,0.001223501],"study_design_scores_gemma":[0.00006235894,0.0002723394,0.006395471,0.00002802664,0.00003999323,0.0003556287,0.00006085214,0.005103935,0.9815718,0.0003264683,0.005744454,0.0000385626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8924941,0.002985873,0.07961061,0.0005254099,0.0002426714,0.000512972,0.002886406,0.001634626,0.01910718],"genre_scores_gemma":[0.8889216,0.004008294,0.05995525,0.000761156,0.0001316121,0.002019742,0.005477797,0.0004743187,0.03825016],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.006036139,"threshold_uncertainty_score":0.02019286,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08277844592996782,"score_gpt":0.3980509421531691,"score_spread":0.3152724962232012,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}