{"id":"W2938872819","doi":"10.1016/bs.mie.2019.03.021","title":"Prenylated FMN: Biosynthesis, purification, and Fdc1 activation","year":2019,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Biochemical and biochemical processes","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Government of Canada; Ontario Genomics Institute; Genome Canada","keywords":"Flavin mononucleotide; Prenyltransferase; Flavin group; Prenylation; Biosynthesis; Cofactor; Biochemistry; Enzyme; Chemistry; Biotransformation; Stereochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004534474,0.001038407,0.0006460577,0.0005704576,0.0006409987,0.0003905165,0.0005059393,0.000425193,0.002245362],"category_scores_gemma":[0.0006800723,0.000405517,0.0003394543,0.0010833,0.0005528827,0.0004564977,0.0005374958,0.001458683,0.001378662],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001672745,"about_ca_system_score_gemma":0.001148835,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.007218558,"about_ca_topic_score_gemma":0.01036944,"domain_scores_codex":[0.9996158,0.00003487024,0.00002778613,0.0001241015,0.00008979196,0.0001076234],"domain_scores_gemma":[0.9997248,0.00007655862,0.00003083769,0.00005767226,0.00005118614,0.000058925],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004614726,0.00006885295,0.0002263148,0.000144728,0.000007522834,0.0000670366,0.0000699389,0.000298077,0.9896585,0.0007066981,0.0004273932,0.007863285],"study_design_scores_gemma":[0.00001791472,0.00004587308,0.001231951,0.000006726448,0.000006014904,0.00009733121,0.00001231904,0.0005015751,0.9904979,0.0001707113,0.007397193,0.00001445961],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8569604,0.005035535,0.1065521,0.001086866,0.0002383132,0.001428671,0.01611476,0.001832332,0.01075099],"genre_scores_gemma":[0.8041463,0.003083173,0.130389,0.0004242411,0.00007182339,0.001032447,0.03863546,0.0004860804,0.02173145],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.007218558,"threshold_uncertainty_score":0.0143531,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02471384269625386,"score_gpt":0.3681638810528107,"score_spread":0.3434500383565569,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}