{"id":"W2940882626","doi":"10.3389/fcimb.2019.00126","title":"Gene Disruption of Honey Bee Trypanosomatid Parasite, Lotmaria passim, by CRISPR/Cas9 System","year":2019,"lang":"en","type":"article","venue":"Frontiers in Cellular and Infection Microbiology","topic":"Insect and Pesticide Research","field":"Agricultural and Biological Sciences","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Xi’an Jiaotong-Liverpool University; McGill University; Harvard T.H. Chan School of Public Health","keywords":"Biology; Honey bee; Gene; CRISPR; Genetics; Cas9; Plasmid; genomic DNA; Electroporation; Genome editing","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002089197,0.0001312584,0.000293359,0.00005081468,0.0000786598,0.00002033679,0.00008724225,0.0002515605,0.0001878258],"category_scores_gemma":[0.00001445533,0.00006392153,0.00006417961,0.0002391608,0.00008699811,0.00008106339,0.00004296877,0.0001602225,0.00002809497],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005145741,"about_ca_system_score_gemma":0.000008145476,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007689295,"about_ca_topic_score_gemma":0.00006692411,"domain_scores_codex":[0.9989083,0.0002238666,0.0002751655,0.0002719676,0.00005424991,0.0002664714],"domain_scores_gemma":[0.9996787,0.00006581977,0.0001056665,0.00005050177,0.00004904603,0.00005028428],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004059388,0.00004522555,0.2266429,0.00004565907,0.00001362134,0.000001029406,0.00002574718,0.000003961927,0.7683832,0.00004333619,0.001744146,0.003010569],"study_design_scores_gemma":[0.001010548,0.001035457,0.1214714,0.00008707136,0.00002992608,0.00007163895,0.0002989544,0.0007727102,0.8570905,0.0001927514,0.0175097,0.0004293626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970022,0.0008682013,0.0006202924,0.00005796272,0.0007004278,0.0002795928,0.00007766551,0.00002678995,0.000366823],"genre_scores_gemma":[0.9989866,0.0002627049,0.0001188446,0.00002671714,0.0000774163,0.00001048764,0.0003084272,0.000001627851,0.0002071585],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1051715,"threshold_uncertainty_score":0.2606644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006527861880701496,"score_gpt":0.2077037021376034,"score_spread":0.2011758402569019,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}