{"id":"W2940882626","doi":"10.3389/fcimb.2019.00126","title":"Gene Disruption of Honey Bee Trypanosomatid Parasite, Lotmaria passim, by CRISPR/Cas9 System","year":2019,"lang":"en","type":"article","venue":"Frontiers in Cellular and Infection Microbiology","topic":"Insect and Pesticide Research","field":"Agricultural and Biological Sciences","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Xi’an Jiaotong-Liverpool University; McGill University; Harvard T.H. Chan School of Public Health","keywords":"Biology; Honey bee; Gene; CRISPR; Genetics; Cas9; Plasmid; genomic DNA; Electroporation; Genome editing","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001246447,0.0003132898,0.0002669221,0.000260727,0.0001922137,0.0002629892,0.000359193,0.000388884,0.001005631],"category_scores_gemma":[0.0001374256,0.0001672832,0.0002877739,0.0001217913,0.0002099816,0.0001346092,0.0003356785,0.0004556578,0.0003902395],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002886059,"about_ca_system_score_gemma":0.0001904723,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009041138,"about_ca_topic_score_gemma":0.001750403,"domain_scores_codex":[0.9998933,0.00001106261,0.00001706036,0.00003434364,0.00002796966,0.00001622407],"domain_scores_gemma":[0.9998976,0.00001665793,0.00004659081,0.00001361652,0.000009448479,0.00001612771],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002466234,0.00001038872,0.0002844045,0.00005463456,0.00000486875,0.0001009325,0.00003704937,0.00007011284,0.9976457,0.0001401304,0.00006595354,0.001561292],"study_design_scores_gemma":[0.0000155644,0.0001688936,0.006264519,0.00001716868,0.00003485709,0.000674438,0.00006518513,0.003117532,0.980832,0.00005767784,0.008738429,0.00001368068],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.974068,0.0006789472,0.01953254,0.0001969336,0.00005777742,0.000167921,0.00124233,0.00068624,0.00336911],"genre_scores_gemma":[0.9741488,0.0004502137,0.01761261,0.00008030747,0.000008119202,0.0001476654,0.001554979,0.00006862848,0.005928698],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001005631,"threshold_uncertainty_score":0.003364146,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006527861880701496,"score_gpt":0.2077037021376034,"score_spread":0.2011758402569019,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}