{"id":"W2942044904","doi":"10.1021/acscentsci.9b00174","title":"Uptake and Fate of Fluorescently Labeled DNA Nanostructures in Cellular Environments: A Cautionary Tale","year":2019,"lang":"en","type":"article","venue":"ACS Central Science","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":188,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Canada Foundation for Innovation; Canadian Institutes of Health Research; Cancer Research Society; Fundación Alfonso Martín Escudero","keywords":"DNA; Cyanine; Förster resonance energy transfer; Biophysics; Oligonucleotide; Nuclease; Fluorescence; Colocalization; Chemistry; DNA nanotechnology; Cell biology; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001615098,0.000532157,0.0007480782,0.0002780148,0.001018436,0.00111682,0.001024699,0.001633595,0.000949369],"category_scores_gemma":[0.002951243,0.0002841477,0.0006621933,0.0004469195,0.0009763425,0.001680854,0.0009567988,0.002062509,0.0006286816],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006678498,"about_ca_system_score_gemma":0.0005421255,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002218368,"about_ca_topic_score_gemma":0.002907027,"domain_scores_codex":[0.9992054,0.0002053825,0.00008558237,0.0001628053,0.0002429733,0.00009794985],"domain_scores_gemma":[0.9987097,0.0005336149,0.00008264241,0.0002870719,0.0003476612,0.00003939129],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003133384,0.0001032389,0.001704371,0.0020073,0.00007164462,0.001738664,0.001029349,0.001472618,0.8882065,0.01405007,0.01134214,0.07796081],"study_design_scores_gemma":[0.00001160551,0.000225372,0.001393566,0.00009883236,0.00006519275,0.001412983,0.0003431923,0.002659906,0.8903422,0.004524595,0.09885886,0.00006368622],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.4295479,0.1876566,0.2963115,0.03542259,0.01602969,0.0004556652,0.001667398,0.002370296,0.03053841],"genre_scores_gemma":[0.7888891,0.04751231,0.1123556,0.006394762,0.00101078,0.0004297049,0.0007861885,0.0005619033,0.04205975],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002218368,"threshold_uncertainty_score":0.008541524,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003532588537181701,"score_gpt":0.2159227143962514,"score_spread":0.2123901258590697,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}