{"id":"W2942257291","doi":"10.1021/acscentsci.8b00910","title":"Redirecting Pore Assembly of Staphylococcal α-Hemolysin by Protein Engineering","year":2019,"lang":"en","type":"article","venue":"ACS Central Science","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Institutes of Health; Office of Naval Research; University of Texas at Austin; U.S. Department of Energy","keywords":"Proteases; Pore-forming toxin; Proteolysis; Chemistry; Protease; Cytolysis; Metalloproteinase; Cell biology; Receptor; Hemolysin; Chimera (genetics); Fusion protein; Matrix metalloproteinase; Recombinant DNA; Biochemistry; Toxin; Biology; In vitro; Cytotoxicity; Enzyme; Microbial toxins","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001454332,0.0002290518,0.0001157957,0.0001053787,0.00008477231,0.0002065406,0.0001431445,0.0002081449,0.0003361311],"category_scores_gemma":[0.0001062056,0.00007665946,0.000182475,0.00009979415,0.0001784657,0.0001578888,0.0002216817,0.0003935925,0.000169761],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003229549,"about_ca_system_score_gemma":0.0001258393,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002925757,"about_ca_topic_score_gemma":0.0004624287,"domain_scores_codex":[0.9999093,0.00002168299,0.000009349301,0.00001334441,0.00002430753,0.00002201989],"domain_scores_gemma":[0.9999332,0.00001090528,0.00002826181,0.000005845758,0.000007206256,0.00001465564],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002283147,0.00001844994,0.00008385746,0.00001740561,0.000002912667,0.0000435429,0.00001628786,0.0002303855,0.9984939,0.0002150952,0.00002280881,0.0008325317],"study_design_scores_gemma":[0.000009282183,0.00009900145,0.0004872839,0.000003447886,0.000005875374,0.000148229,0.00001495707,0.001651792,0.9952639,0.000056084,0.002257359,0.000002795141],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9842599,0.0004434467,0.01401281,0.0001266712,0.0000281116,0.00005543387,0.00008844531,0.0000651032,0.0009200103],"genre_scores_gemma":[0.9903272,0.0003924581,0.007906135,0.00004596659,0.000004237681,0.00002071341,0.0001133626,0.00001687923,0.001173125],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0003361311,"threshold_uncertainty_score":0.002343178,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004181340095282077,"score_gpt":0.1979108944445836,"score_spread":0.1937295543493015,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}