{"id":"W2942257291","doi":"10.1021/acscentsci.8b00910","title":"Redirecting Pore Assembly of Staphylococcal α-Hemolysin by Protein Engineering","year":2019,"lang":"en","type":"article","venue":"ACS Central Science","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Institutes of Health; Office of Naval Research; University of Texas at Austin; U.S. Department of Energy","keywords":"Proteases; Pore-forming toxin; Proteolysis; Chemistry; Protease; Cytolysis; Metalloproteinase; Cell biology; Receptor; Hemolysin; Chimera (genetics); Fusion protein; Matrix metalloproteinase; Recombinant DNA; Biochemistry; Toxin; Biology; In vitro; Cytotoxicity; Enzyme; Microbial toxins","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003864775,0.0001211983,0.0001818365,0.00007233686,0.0001067237,0.00002150701,0.0004267563,0.00009850747,0.0001747822],"category_scores_gemma":[0.0001137841,0.0001065734,0.00004648856,0.0003941274,0.0001550157,0.0002380819,0.0001144008,0.0002021694,0.0000682647],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005353153,"about_ca_system_score_gemma":0.00009526956,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009037234,"about_ca_topic_score_gemma":0.000001419671,"domain_scores_codex":[0.99876,0.00002468051,0.0002160023,0.0003024446,0.00008681811,0.0006100607],"domain_scores_gemma":[0.9995348,0.00002772652,0.000104156,0.0002469583,0.00005222618,0.00003412278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001188021,0.00004317749,0.0006494663,0.00001018377,0.000009991961,7.227587e-7,0.0001684435,0.00001607263,0.9958867,0.001225225,0.0001023654,0.001875728],"study_design_scores_gemma":[0.0003330577,0.0001136088,0.003780865,0.00004579165,0.000003931316,0.000007738146,0.0001135606,0.00006203139,0.9922076,0.00001602351,0.003185115,0.0001306984],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9978821,0.0003276537,0.000272453,0.00004637388,0.0006277672,0.0002967191,0.00001319363,0.00004808032,0.0004856928],"genre_scores_gemma":[0.9984412,0.000006073803,0.0004835682,0.00002078763,0.000009914525,0.000005721472,0.000009866814,0.00000878468,0.001014131],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003679165,"threshold_uncertainty_score":0.4345936,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004181340095282077,"score_gpt":0.1979108944445836,"score_spread":0.1937295543493015,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}