{"id":"W2944995279","doi":"10.21769/bioprotoc.3242","title":"Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells","year":2019,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Adenosine and Purinergic Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Shriners Hospitals for Children - Canada; McGill University","funders":"Canadian Institutes of Health Research; Réseau de Recherche en Santé Buccodentaire et Osseuse; Faculty of Dentistry, McGill University; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Extracellular; Intracellular; Purinergic receptor; Cell biology; Calcium in biology; Calcium; Biochemistry; Biophysics; Biology; Luciferase; Chemistry; Receptor; Luciferin; Transfection","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002057842,0.0002012093,0.0002285016,0.00006632251,0.00002559505,0.00001751364,0.0001760272,0.0002199306,0.0001518882],"category_scores_gemma":[0.00003125078,0.0001875248,0.00007027958,0.0001044728,0.00006325337,0.000004791692,0.0001217029,0.0001181524,0.00002299299],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001499204,"about_ca_system_score_gemma":0.00004559904,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001082367,"about_ca_topic_score_gemma":0.000003507524,"domain_scores_codex":[0.9987292,0.00005474584,0.0003842544,0.0004101675,0.0001372707,0.0002843432],"domain_scores_gemma":[0.9993483,0.00002361548,0.0001470671,0.0003345936,0.00005804102,0.00008836191],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003248267,0.00009799878,0.005492001,0.0002287199,0.00001343964,0.00003342515,0.00005931804,0.0002272914,0.9911053,0.00002687015,0.0009003087,0.001490554],"study_design_scores_gemma":[0.001413219,0.0004002766,0.0001626734,0.00005507654,0.000006198744,0.00001004612,0.00005832663,0.0005976557,0.8201458,0.00001537747,0.176917,0.0002182284],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8038055,0.0001939353,0.0004017665,0.00007260693,0.00008804869,0.1951263,0.00001152636,0.00001371925,0.00028666],"genre_scores_gemma":[0.9063765,0.000004947608,0.001020241,0.00007287411,0.0001872554,0.09062047,0.00002092738,0.00004247911,0.001654294],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1760167,"threshold_uncertainty_score":0.7647038,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00998516775713065,"score_gpt":0.2602342676591082,"score_spread":0.2502490999019776,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}