{"id":"W2948330247","doi":"10.1111/febs.14954","title":"Semi‐rational evolution of the 3‐(3‐hydroxyalkanoyloxy)alkanoate (<scp>HAA</scp>) synthase RhlA to improve rhamnolipid production in <i>Pseudomonas aeruginosa</i> and <i>Burkholderia glumae</i>","year":2019,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Microbial bioremediation and biosurfactants","field":"Environmental Science","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; PROTEO; Institut National de la Recherche Scientifique","funders":"National Institute of General Medical Sciences; Fonds de Recherche du Québec - Santé; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; National Institutes of Health; Fonds de recherche du Québec – Nature et technologies; Armand-Frappier Foundation","keywords":"Burkholderia; Chemistry; Mutant; Pseudomonas aeruginosa; Rational design; Mutagenesis; Computational biology; Biochemistry; Microbiology; Bacteria; Biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007474723,0.0001985644,0.0002176882,0.00008946122,0.0001616664,0.00006952316,0.0002825637,0.0001180083,0.0003151818],"category_scores_gemma":[0.0002439104,0.0001483406,0.000078465,0.0004702161,0.000131041,0.0004206719,0.0001920124,0.0003362762,0.0002167777],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003379528,"about_ca_system_score_gemma":0.00007797858,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009440847,"about_ca_topic_score_gemma":0.0001690833,"domain_scores_codex":[0.9980651,0.0001389617,0.0005144256,0.0003794922,0.0005472229,0.0003547595],"domain_scores_gemma":[0.9991267,0.0000491006,0.0003493176,0.0002641739,0.00004417582,0.0001665345],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002543004,0.00008250902,0.1105029,0.00001096472,0.00000800436,0.00000153361,0.0003403311,0.0005599838,0.8821252,0.00002126862,0.005529726,0.0007921671],"study_design_scores_gemma":[0.001258108,0.0001665914,0.3411126,0.0001210723,0.00002374234,0.00021877,0.0002756829,0.0003177653,0.6474189,0.0003761135,0.00851662,0.000194073],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9957156,0.00007271738,0.00004726504,0.0009827551,0.001442426,0.0005355788,0.00003139696,0.00001373751,0.001158549],"genre_scores_gemma":[0.9978491,0.00005706113,0.0002932155,0.0003234334,0.0002017217,0.000004914194,0.000003803751,0.00001482099,0.001251935],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2347063,"threshold_uncertainty_score":0.6049154,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004341154707678276,"score_gpt":0.1894540524217475,"score_spread":0.1851128977140692,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}