{"id":"W2948330247","doi":"10.1111/febs.14954","title":"Semi‐rational evolution of the 3‐(3‐hydroxyalkanoyloxy)alkanoate (<scp>HAA</scp>) synthase RhlA to improve rhamnolipid production in <i>Pseudomonas aeruginosa</i> and <i>Burkholderia glumae</i>","year":2019,"lang":"en","type":"article","venue":"FEBS Journal","topic":"Microbial bioremediation and biosurfactants","field":"Environmental Science","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; PROTEO; Institut National de la Recherche Scientifique","funders":"National Institute of General Medical Sciences; Fonds de Recherche du Québec - Santé; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; National Institutes of Health; Fonds de recherche du Québec – Nature et technologies; Armand-Frappier Foundation","keywords":"Burkholderia; Chemistry; Mutant; Pseudomonas aeruginosa; Rational design; Mutagenesis; Computational biology; Biochemistry; Microbiology; Bacteria; Biology; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002118456,0.0004496646,0.0003454587,0.0001371476,0.0001238738,0.0003595985,0.0003743532,0.0003111087,0.0003785044],"category_scores_gemma":[0.0002777091,0.0001359944,0.0003389277,0.0002448773,0.0001854383,0.0002079251,0.000366135,0.0004279975,0.0002109068],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00037769,"about_ca_system_score_gemma":0.0004046782,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00118377,"about_ca_topic_score_gemma":0.001285733,"domain_scores_codex":[0.9998647,0.00003095885,0.00001191948,0.00002396528,0.0000407541,0.00002765683],"domain_scores_gemma":[0.9999207,0.00002152382,0.00002561365,0.000009257835,0.0000122463,0.00001058787],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000163707,0.0001405453,0.000663101,0.0001083053,0.00002949763,0.0002301801,0.00006326675,0.01291213,0.976555,0.000650792,0.00008417642,0.008399215],"study_design_scores_gemma":[0.00003607942,0.0009475093,0.002211894,0.00001936596,0.00006478175,0.0003789303,0.0001313982,0.05786444,0.9339746,0.000205929,0.004138232,0.00002675147],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.978663,0.0005227592,0.01897585,0.000084343,0.00001648419,0.00009048718,0.0002245291,0.0001182837,0.001304273],"genre_scores_gemma":[0.9840036,0.0004654138,0.01449196,0.00002725579,0.00000200982,0.00004426786,0.000258025,0.00004910733,0.0006584175],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00118377,"threshold_uncertainty_score":0.002740324,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004341154707678276,"score_gpt":0.1894540524217475,"score_spread":0.1851128977140692,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}