{"id":"W2948466000","doi":"10.1002/slct.201901762","title":"Lost in Translation: Challenges with Heterologous Expression of Lichen Polyketide Synthases","year":2019,"lang":"en","type":"article","venue":"ChemistrySelect","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Winnipeg; University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Polyketide synthase; Polyketide; Biology; Aspergillus oryzae; Gene; Heterologous expression; Heterologous; Genetics; Biochemistry; Biosynthesis; Enzyme; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003660537,0.0006460934,0.0009735581,0.0003498246,0.0004485727,0.002480592,0.0007366518,0.0007304171,0.001264544],"category_scores_gemma":[0.002530504,0.000487419,0.0007049026,0.0004373325,0.0006526218,0.0009068595,0.00119238,0.002565982,0.0020806],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009290582,"about_ca_system_score_gemma":0.0006510079,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006749236,"about_ca_topic_score_gemma":0.001145887,"domain_scores_codex":[0.9976074,0.0006061314,0.0004493269,0.0002923151,0.0008178416,0.0002270969],"domain_scores_gemma":[0.9972028,0.001292816,0.0002815878,0.0005137273,0.00053882,0.0001702801],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006125775,0.00005505986,0.0002727477,0.0001597325,0.00001110551,0.00009903216,0.0001343854,0.0002508145,0.9948049,0.0004879727,0.0002234312,0.003439475],"study_design_scores_gemma":[0.00002423871,0.000305177,0.001177884,0.00004795994,0.00004335142,0.0005701853,0.0003426083,0.003842498,0.9783754,0.0005250152,0.0147255,0.0000201668],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9134036,0.00500134,0.06988071,0.003879777,0.0006834118,0.0003913114,0.001125587,0.0007817564,0.004852506],"genre_scores_gemma":[0.8902295,0.004611325,0.07764281,0.001152431,0.0002668711,0.0006391857,0.008250076,0.001211183,0.01599663],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003660537,"threshold_uncertainty_score":0.01935899,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01691265025169049,"score_gpt":0.2324424757319736,"score_spread":0.2155298254802831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}