{"id":"W2948466000","doi":"10.1002/slct.201901762","title":"Lost in Translation: Challenges with Heterologous Expression of Lichen Polyketide Synthases","year":2019,"lang":"en","type":"article","venue":"ChemistrySelect","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Winnipeg; University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Polyketide synthase; Polyketide; Biology; Aspergillus oryzae; Gene; Heterologous expression; Heterologous; Genetics; Biochemistry; Biosynthesis; Enzyme; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006364971,0.0001176393,0.0002669199,0.00003664777,0.000008696682,0.000003096354,0.00006721796,0.0001013598,0.00029194],"category_scores_gemma":[0.00004322172,0.000079611,0.00004055589,0.000108846,0.00003997875,0.00003284429,0.000009968742,0.0001430944,0.000007961017],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001961203,"about_ca_system_score_gemma":0.00003818067,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001151797,"about_ca_topic_score_gemma":0.000009069754,"domain_scores_codex":[0.9993103,0.00001270384,0.0001580952,0.0002491587,0.0001148094,0.0001549369],"domain_scores_gemma":[0.9995714,0.00005490932,0.0000621646,0.000222536,0.00004750343,0.00004148611],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004921107,0.0001236236,0.003585473,0.000446024,0.00001454723,0.000008647384,0.0001103262,0.000001272739,0.9904227,5.747112e-7,0.00007581601,0.004718899],"study_design_scores_gemma":[0.0006631866,0.0001967846,0.003950999,0.0004609817,0.00002188908,0.00005575573,0.00006641631,0.000005128769,0.9939224,0.000003922588,0.0005583813,0.00009413716],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9920616,0.003665725,7.464805e-7,0.001090877,0.00001389523,0.0001841392,0.000007311764,0.00001722483,0.002958459],"genre_scores_gemma":[0.99897,0.0002786478,0.0003045652,0.00004792145,0.00006562972,7.07062e-7,0.0000189758,0.0000125235,0.0003010831],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006908325,"threshold_uncertainty_score":0.3246442,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01691265025169049,"score_gpt":0.2324424757319736,"score_spread":0.2155298254802831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}