{"id":"W2949222337","doi":"10.1002/bit.27084","title":"Construction of a sensitive pyrogen‐testing cell model by site‐specific knock‐in of multiple genes","year":2019,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; National Natural Science Foundation of China","keywords":"TLR4; CD14; Chemistry; Cell biology; Molecular biology; Biology; Signal transduction; Flow cytometry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009651557,0.0009733356,0.000873111,0.0006695738,0.0003892262,0.0007456871,0.0009256706,0.0009554497,0.001587069],"category_scores_gemma":[0.0004461537,0.0005020928,0.0007442562,0.0003126841,0.0005738044,0.0006753091,0.0007309616,0.001868626,0.001104227],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004017301,"about_ca_system_score_gemma":0.0004015406,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005033802,"about_ca_topic_score_gemma":0.0007355189,"domain_scores_codex":[0.9987809,0.0001586024,0.0001773824,0.0002574766,0.0004965879,0.0001290065],"domain_scores_gemma":[0.9992856,0.0001402608,0.0001780988,0.0001847901,0.0001063282,0.0001048111],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000346784,0.00004135143,0.0001562646,0.00003474434,0.000007580742,0.00005648784,0.00002041622,0.0001646825,0.9980626,0.000358739,0.0001081228,0.0009543985],"study_design_scores_gemma":[0.00001990644,0.0002157084,0.0006244983,0.000007958421,0.00003367525,0.0002261018,0.00002328671,0.003381092,0.9897811,0.0001231149,0.00554774,0.00001579741],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5328037,0.001595562,0.4534227,0.0005934998,0.0005213851,0.0009655205,0.003390209,0.001925822,0.004781644],"genre_scores_gemma":[0.8132342,0.001960275,0.1644612,0.0003992984,0.00006898589,0.001618783,0.005607615,0.0003218928,0.01232777],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001587069,"threshold_uncertainty_score":0.005309284,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005065957138799542,"score_gpt":0.207681418721801,"score_spread":0.2026154615830014,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}