{"id":"W2953327680","doi":"10.1016/j.jsb.2019.06.003","title":"A surrogate structural platform informed by ancestral reconstruction and resurrection of a putative carbohydrate binding module hybrid illuminates the neofunctionalization of a pectate lyase","year":2019,"lang":"en","type":"article","venue":"Journal of Structural Biology","topic":"Polysaccharides and Plant Cell Walls","field":"Agricultural and Biological Sciences","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Lethbridge; University of Victoria; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada","keywords":"Pectate lyase; Biology; Gene; Biochemistry; Computational biology; Yersinia enterocolitica; Genome; Lyase; Horizontal gene transfer; Genetics; Structural motif; Enzyme; Bacteria; Pectinase","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003444354,0.000353077,0.000274978,0.00027415,0.0005960856,0.0007782432,0.000589062,0.000726875,0.004011482],"category_scores_gemma":[0.0007002598,0.0003113131,0.0004081902,0.0003380627,0.0003871829,0.000497227,0.0007989762,0.001706402,0.0009815921],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004439462,"about_ca_system_score_gemma":0.0004186406,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006788048,"about_ca_topic_score_gemma":0.001282155,"domain_scores_codex":[0.9999,0.00001868551,0.000003805011,0.00003200471,0.00002258113,0.00002299335],"domain_scores_gemma":[0.9998492,0.00004039219,0.00001828391,0.00004453055,0.0000154115,0.00003226172],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007633179,0.0001483134,0.004303463,0.0001484756,0.00007273953,0.00128792,0.0003521969,0.02296846,0.9099687,0.0314554,0.0009803005,0.02755062],"study_design_scores_gemma":[0.0001788509,0.0009752211,0.01315865,0.00009495371,0.000253134,0.001769635,0.0005525003,0.3884551,0.5113794,0.03133912,0.0516588,0.000184634],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9186091,0.000216866,0.0741539,0.0002902541,0.0001604641,0.00003156803,0.0006498745,0.0008745852,0.005013292],"genre_scores_gemma":[0.9693145,0.0001470706,0.02773565,0.00006948828,0.000013077,0.00001832714,0.000940184,0.0003437873,0.001418013],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004011482,"threshold_uncertainty_score":0.01341975,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01115922112067957,"score_gpt":0.2222436114795507,"score_spread":0.2110843903588711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}