{"id":"W2953875568","doi":"10.1007/s00792-019-01105-8","title":"Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2AT for chitin extraction from crab and shrimp shell by-products","year":2019,"lang":"en","type":"article","venue":"Extremophiles","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Ministère de l'Enseignement Supérieur, de la Recherche, de la Science et de la Technologie","keywords":"PMSF; Chitin; Shrimp; Subtilisin; Biochemistry; Serine protease; Litopenaeus; Biology; Proteases; Portunus trituberculatus; Protease; Chromatography; Chemistry; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001896089,0.0005475665,0.0002491822,0.0001925977,0.0001409723,0.0002646266,0.0001571501,0.0001826417,0.000340771],"category_scores_gemma":[0.0002105487,0.0001504856,0.0003493626,0.0002400578,0.0001615307,0.0002739203,0.0002083719,0.0004217763,0.0002542512],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002669878,"about_ca_system_score_gemma":0.00025338,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001284658,"about_ca_topic_score_gemma":0.00151066,"domain_scores_codex":[0.9998864,0.00001659011,0.00001223579,0.0000231902,0.00004111673,0.00002042819],"domain_scores_gemma":[0.9998462,0.00002550486,0.00003555093,0.00001777974,0.00003311493,0.00004185936],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002630374,0.00001473236,0.0001747369,0.00001651526,0.000002962108,0.00004106383,0.00001318743,0.00004029779,0.9989316,0.0000153776,0.00001319788,0.0007099638],"study_design_scores_gemma":[0.000009631807,0.0001118357,0.01060929,0.000004632052,0.00001554608,0.0004926247,0.00003968942,0.0008046426,0.9858966,0.00003731987,0.001971486,0.000006681194],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9894676,0.00101979,0.007715404,0.0001789909,0.00002492839,0.00004174409,0.00066861,0.00003623471,0.0008468135],"genre_scores_gemma":[0.9824973,0.0007555669,0.008849734,0.00007901915,0.00001834201,0.00003273864,0.004040858,0.00002958897,0.003696919],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001284658,"threshold_uncertainty_score":0.002554357,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009796978017043458,"score_gpt":0.2164272768442038,"score_spread":0.2066302988271604,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}