{"id":"W2953875568","doi":"10.1007/s00792-019-01105-8","title":"Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2AT for chitin extraction from crab and shrimp shell by-products","year":2019,"lang":"en","type":"article","venue":"Extremophiles","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Ministère de l'Enseignement Supérieur, de la Recherche, de la Science et de la Technologie","keywords":"PMSF; Chitin; Shrimp; Subtilisin; Biochemistry; Serine protease; Litopenaeus; Biology; Proteases; Portunus trituberculatus; Protease; Chromatography; Chemistry; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001203452,0.0002032548,0.000214821,0.00004117261,0.00006744079,0.00004935468,0.00006614443,0.0001731277,0.00002260806],"category_scores_gemma":[0.00006949739,0.0001958086,0.00002722023,0.00007062739,0.00008553607,0.00003863833,0.00005368982,0.00006984398,0.000001090487],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009342686,"about_ca_system_score_gemma":0.00002729099,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006301931,"about_ca_topic_score_gemma":0.000002637689,"domain_scores_codex":[0.9987944,0.00003972791,0.0002820942,0.0006335785,0.0001041808,0.0001460257],"domain_scores_gemma":[0.999272,0.00001834126,0.0002249181,0.0002872544,0.0001303708,0.00006713546],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004632629,0.0001308036,0.0009155902,0.00007561689,0.00003420872,1.599505e-7,0.0001567359,0.000001285263,0.9950612,0.00002516382,0.00005331688,0.003082647],"study_design_scores_gemma":[0.001283033,0.0001268918,0.03599479,0.00004553177,0.00003217942,0.00001064343,0.00005867758,0.00123495,0.9575247,0.00007104898,0.003394172,0.0002234533],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9936183,0.001325543,0.002266685,0.001040795,0.0001195838,0.0009453287,0.000649179,0.00001957142,0.00001500923],"genre_scores_gemma":[0.9931737,0.0006619283,0.001326104,0.0000971635,0.0002641437,0.00005628702,0.004183777,0.00003479979,0.0002020622],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03753658,"threshold_uncertainty_score":0.7984842,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009796978017043458,"score_gpt":0.2164272768442038,"score_spread":0.2066302988271604,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}