{"id":"W2956128574","doi":"10.1016/j.plasmid.2019.102419","title":"Assembly of large mobilizable genetic cargo by double recombinase operated insertion of DNA (DROID)","year":2019,"lang":"en","type":"article","venue":"Plasmid","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"Centre Hospitalier Universitaire de Sherbrooke; Université de Sherbrooke","funders":"Fonds de recherche du Québec – Nature et technologies; Canadian Institutes of Health Research; Université de Sherbrooke","keywords":"Plasmid; Biology; Recombinase; CRISPR; DNA; Cas9; Trans-activating crRNA; Genetics; Cre recombinase; Computational biology; Insert (composites); Gene; Transgene; Recombination","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000260867,0.0004467551,0.0003655832,0.0003647973,0.0003347627,0.0007091137,0.0003605705,0.0003043877,0.001560275],"category_scores_gemma":[0.0001902464,0.000327377,0.0003509884,0.0002819045,0.0002720633,0.0003394006,0.0006660745,0.0008811728,0.001284355],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006638207,"about_ca_system_score_gemma":0.0002906237,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006131209,"about_ca_topic_score_gemma":0.001160008,"domain_scores_codex":[0.9998338,0.00001339204,0.00001719243,0.00004996048,0.00004651414,0.00003910106],"domain_scores_gemma":[0.9998716,0.0000140909,0.00002909769,0.00002318455,0.00001536664,0.00004659129],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004336564,0.0000229065,0.0001538563,0.00003152575,0.00000620883,0.00008265502,0.00005430475,0.0001246667,0.9974514,0.0004802974,0.0001445539,0.001404325],"study_design_scores_gemma":[0.00001043681,0.00004355432,0.0005230166,0.000004405027,0.000008374992,0.0001079668,0.00003253484,0.00133352,0.991945,0.00006931418,0.005913919,0.000007937779],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9125675,0.0006669806,0.07893611,0.0002868826,0.000182989,0.00021462,0.001105368,0.001178845,0.004860836],"genre_scores_gemma":[0.9454395,0.0004015849,0.03539293,0.00009035807,0.00001187474,0.00008256372,0.002353201,0.0003660694,0.01586199],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001560275,"threshold_uncertainty_score":0.005219638,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004766488571567525,"score_gpt":0.2601829411904596,"score_spread":0.2554164526188921,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}