{"id":"W2958332578","doi":"10.1101/700252","title":"Streamlined production, purification, and comparison of recombinant extracellular polyhydroxybutyrate depolymerases","year":2019,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Agriculture and Forestry","keywords":"Comamonas testosteroni; Pseudomonas stutzeri; Recombinant DNA; Polyhydroxybutyrate; Chemistry; Heterologous expression; Escherichia coli; Ralstonia; Heterologous; Polyhydroxyalkanoates; Biochemistry; Pseudomonas putida; Bacteria; Microbiology; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008096363,0.0007097046,0.0004790889,0.0004310614,0.0001545077,0.0007075469,0.0003086884,0.0003862145,0.0003019644],"category_scores_gemma":[0.0006624316,0.0002058613,0.0004277921,0.0006242706,0.0002729729,0.0003490675,0.0003976606,0.0006606911,0.0004499369],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003575292,"about_ca_system_score_gemma":0.0003052558,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009279922,"about_ca_topic_score_gemma":0.001037365,"domain_scores_codex":[0.9992932,0.0001122571,0.0001257455,0.0001115713,0.0002955219,0.00006161675],"domain_scores_gemma":[0.9996707,0.00006641331,0.00008442067,0.00006455724,0.0000806677,0.00003321766],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001403316,0.00002078418,0.00009811877,0.00004259271,0.000006111446,0.00001457695,0.000008000324,0.0001006432,0.9984736,0.00003196463,0.00001263744,0.001176965],"study_design_scores_gemma":[0.000003437508,0.00005223328,0.0007899603,0.000003197532,0.00001031067,0.00006231583,0.000009875285,0.0004565556,0.9977283,0.00001551424,0.0008649918,0.000003309122],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9115496,0.002471388,0.08192506,0.0002551897,0.00008197488,0.0002801123,0.001761969,0.0003319372,0.001342877],"genre_scores_gemma":[0.8974394,0.003937536,0.08868188,0.00007546537,0.00003513085,0.0002326759,0.006222293,0.0001831452,0.003192587],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009279922,"threshold_uncertainty_score":0.004281819,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02384323387266023,"score_gpt":0.2271110749364864,"score_spread":0.2032678410638262,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}