{"id":"W2964564658","doi":"10.1038/s41598-019-47629-z","title":"Gene-edited murine cell lines for propagation of chronic wasting disease prions","year":2019,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Alberta Prion Research Institute; Genome Alberta; Canada Research Chairs; Alberta Agriculture and Forestry; Genome Canada","keywords":"Chronic wasting disease; Bank vole; Subcloning; Biology; Virology; Transduction (biophysics); Cell culture; Bovine spongiform encephalopathy; Cell; CRISPR; Gene; Neurodegeneration; Cell biology; Disease; Prion protein; Genetics; Scrapie; Plasmid; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001027287,0.0009813836,0.0005146897,0.001471878,0.0005620179,0.0007032432,0.001130706,0.001183549,0.00593876],"category_scores_gemma":[0.0004889749,0.0005198838,0.0008140827,0.0006357945,0.0006006065,0.0005222002,0.0006013801,0.002339405,0.003769831],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004945542,"about_ca_system_score_gemma":0.0004614435,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007269952,"about_ca_topic_score_gemma":0.001437821,"domain_scores_codex":[0.9989581,0.0001398531,0.0002209949,0.0002428912,0.0003105903,0.0001275943],"domain_scores_gemma":[0.999095,0.0001977287,0.0001640219,0.0002609056,0.0001366411,0.0001458042],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001169368,0.0001325427,0.0002488287,0.00008960361,0.00001525248,0.0001536294,0.0001105588,0.0002449361,0.9952384,0.001144996,0.0005049568,0.001999228],"study_design_scores_gemma":[0.0001110376,0.0005778741,0.001694979,0.00005844242,0.00006109136,0.0007593411,0.00004332745,0.002310582,0.9732739,0.0003036984,0.02078032,0.00002540208],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7771845,0.004844299,0.1699443,0.000877296,0.0009837395,0.002307121,0.02524141,0.002435823,0.01618146],"genre_scores_gemma":[0.7740148,0.006158393,0.144482,0.0004505143,0.0001575673,0.003346149,0.02797441,0.0009921523,0.04242393],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00593876,"threshold_uncertainty_score":0.01986718,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008356656941395035,"score_gpt":0.2443941136487331,"score_spread":0.2360374567073381,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}