{"id":"W2965868647","doi":"10.1016/j.jbiotec.2019.07.009","title":"Site-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus","year":2019,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Genome Canada","keywords":"Succinic acid; Carboxylate; Chemistry; Biochemistry; Mutagenesis; Stereochemistry; Site-directed mutagenesis; Reductase; Alanine; Carboxylic acid; Mutant; Enzyme; Active site; Protein engineering; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001737981,0.0001003291,0.0002202906,0.00005961787,0.00001956915,0.000005505857,0.0001806916,0.0002782756,0.00002358389],"category_scores_gemma":[0.0001154984,0.00007010899,0.00008147475,0.0001353612,0.0001141578,0.000003701415,0.00009848743,0.0001668323,0.000001086992],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001036796,"about_ca_system_score_gemma":0.00003822332,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003721651,"about_ca_topic_score_gemma":0.000007984415,"domain_scores_codex":[0.9993247,0.0000487911,0.000270059,0.0001727097,0.00007065067,0.0001130351],"domain_scores_gemma":[0.999266,0.000004072519,0.0002574225,0.0003623474,0.00008252561,0.00002766058],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008052288,0.00002960551,0.002500959,0.00002065021,0.00005917587,5.990715e-7,0.0000273578,0.00000324292,0.9952788,0.000006069613,0.00009757293,0.001895458],"study_design_scores_gemma":[0.0002610703,0.000164941,0.01081695,0.000016477,0.00003981883,0.00004346131,0.00004918069,0.000004907527,0.9828414,0.00001383073,0.005674971,0.00007302988],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950941,0.003314127,0.00003058709,0.0006286501,0.0007833174,0.00008631317,0.00004447278,0.00001057992,0.000007842314],"genre_scores_gemma":[0.998543,0.0005845174,0.000627975,0.00001496665,0.0001778384,5.767321e-7,0.000006916148,0.000009152349,0.0000350311],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01243742,"threshold_uncertainty_score":0.2858962,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003330270305729129,"score_gpt":0.1823879383073072,"score_spread":0.1790576680015781,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}