{"id":"W2965868647","doi":"10.1016/j.jbiotec.2019.07.009","title":"Site-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus","year":2019,"lang":"en","type":"article","venue":"Journal of Biotechnology","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Genome Canada","keywords":"Succinic acid; Carboxylate; Chemistry; Biochemistry; Mutagenesis; Stereochemistry; Site-directed mutagenesis; Reductase; Alanine; Carboxylic acid; Mutant; Enzyme; Active site; Protein engineering; Amino acid","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003441262,0.0002804355,0.0003302361,0.0002588678,0.0002264458,0.0003014989,0.0004073624,0.0003433299,0.0009893441],"category_scores_gemma":[0.0004340385,0.0001864516,0.0003324299,0.0002990932,0.0002246112,0.000128877,0.0002461082,0.000487504,0.0003374094],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004653018,"about_ca_system_score_gemma":0.0004153324,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002220425,"about_ca_topic_score_gemma":0.00178534,"domain_scores_codex":[0.9996839,0.00008547714,0.00003866656,0.00002576723,0.0001127229,0.00005361372],"domain_scores_gemma":[0.9997718,0.00005602586,0.00006034212,0.00002586829,0.00005763181,0.00002832851],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001265357,0.00006394046,0.0004857047,0.0000216365,0.00001104214,0.00005875861,0.0000246548,0.0001955237,0.9976951,0.0001238809,0.00003271726,0.00116045],"study_design_scores_gemma":[0.00001931764,0.0002275487,0.003524139,0.000005524008,0.00002334805,0.0002525559,0.00004277484,0.001295692,0.99365,0.00004245507,0.0009082655,0.000008395398],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981178,0.0002230076,0.00110609,0.00006309294,0.00001450571,0.00001109744,0.0001527836,0.00001858181,0.0002930932],"genre_scores_gemma":[0.9976305,0.0001356842,0.001075866,0.00002573609,0.000004733292,0.000008376373,0.0003683563,0.00002401287,0.0007266201],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002220425,"threshold_uncertainty_score":0.004415035,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003330270305729129,"score_gpt":0.1823879383073072,"score_spread":0.1790576680015781,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}