{"id":"W2966141646","doi":"10.1073/pnas.1902889116","title":"Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site","year":2019,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Cancer Institute; National Institutes of Health; National Institute of General Medical Sciences; Österreichische Forschungsförderungsgesellschaft; Deutsche Forschungsgemeinschaft; European Commission; American Lebanese Syrian Associated Charities; Genome Canada; Austrian Science Fund; Translation Centre for the Bodies of the European Union; Howard Hughes Medical Institute","keywords":"Ubiquitin ligase; Biochemistry; Ubiquitin; Ubiquitin-Protein Ligases; Binding site; DNA ligase; Biology; Protein–protein interaction; Protein subunit; Plasma protein binding; Enzyme; Chemistry; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008902692,0.0002959142,0.0002005275,0.0001503324,0.0001615342,0.0003041551,0.00032437,0.0002157142,0.0005628568],"category_scores_gemma":[0.0001141895,0.0001388883,0.0001934681,0.0001348009,0.0001791002,0.0000921863,0.0001391897,0.0004143841,0.0002596049],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004106121,"about_ca_system_score_gemma":0.0002570143,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001451625,"about_ca_topic_score_gemma":0.001433319,"domain_scores_codex":[0.9998901,0.00001077445,0.0000097038,0.00002398862,0.00004441633,0.00002097157],"domain_scores_gemma":[0.9999279,0.00001001197,0.00002725429,0.000007880732,0.000008814217,0.00001812566],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005888028,0.00003000155,0.0001857498,0.00001284787,0.000005130712,0.00006134984,0.000004921131,0.0005494722,0.9978636,0.0002213082,0.00004533137,0.0009614514],"study_design_scores_gemma":[0.000006269325,0.00007227565,0.0006449296,0.000001381478,0.000007567351,0.0001570129,0.000004948904,0.00316491,0.9941222,0.0000260701,0.001789668,0.000002757852],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9842352,0.0005050666,0.01218402,0.0001055302,0.00002446436,0.00005761802,0.0004133514,0.0001301005,0.002344649],"genre_scores_gemma":[0.9874656,0.0003245908,0.008909851,0.00003621965,0.000004868551,0.00002356372,0.0004777389,0.00004482385,0.002712825],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001451625,"threshold_uncertainty_score":0.002979219,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01700090508540554,"score_gpt":0.2509645567897706,"score_spread":0.2339636517043651,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}