{"id":"W2967350153","doi":"10.1038/s41467-019-11588-w","title":"Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"Plant tissue culture and regeneration","field":"Biochemistry, Genetics and Molecular Biology","cited_by":102,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Natural Sciences and Engineering Research Council of Canada; National Institutes of Health","keywords":"Yeast; Tropane; Biosynthesis; Saccharomyces cerevisiae; Metabolic engineering; Synthetic biology; Bacteria; Biochemistry; Biology; Fermentation; Gene; Chemistry; Computational biology; Stereochemistry; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002394356,0.0004798124,0.0002630627,0.0002750889,0.0001560914,0.0004797758,0.0002893026,0.0002871,0.0004550037],"category_scores_gemma":[0.0002099774,0.0001871501,0.0003203532,0.0003810482,0.0001609895,0.0002985582,0.0004961885,0.0007019907,0.0004395286],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002615332,"about_ca_system_score_gemma":0.0004173339,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007686829,"about_ca_topic_score_gemma":0.001370688,"domain_scores_codex":[0.9998198,0.00002688728,0.00002276935,0.00003511197,0.00006176681,0.00003376137],"domain_scores_gemma":[0.999897,0.00001536802,0.00003105394,0.00001654352,0.00001652671,0.0000234511],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002270508,0.00003074899,0.0001089388,0.00003273987,0.000005137147,0.00005700684,0.00001319369,0.0002833993,0.9977117,0.0001629966,0.00002420779,0.001547285],"study_design_scores_gemma":[0.00001153597,0.0001699272,0.0007294157,0.000008921901,0.00001569946,0.0001333222,0.00003496672,0.002416957,0.9928231,0.00008565723,0.003561767,0.000008613895],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9340038,0.0009673029,0.05971666,0.0002517547,0.0000850021,0.0001910047,0.0009908591,0.0005632129,0.003230318],"genre_scores_gemma":[0.9205098,0.001555507,0.07386842,0.00005158049,0.00001217482,0.0001079905,0.001654677,0.0001300011,0.002109959],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007686829,"threshold_uncertainty_score":0.001897573,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01029279741789582,"score_gpt":0.2452046183037663,"score_spread":0.2349118208858705,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}