{"id":"W2967671156","doi":"10.1038/s41467-019-11673-0","title":"An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":60,"is_retracted":false,"has_abstract":true,"ca_institutions":"Wilfrid Laurier University","funders":"National Institute of Dental and Craniofacial Research; National Natural Science Foundation of China; Mikrobiologický Ústav, Akademie Věd České Republiky; University of Oklahoma Health Sciences Center; University of Massachusetts Amherst; University of Oklahoma","keywords":"Heterologous expression; Heterologous; Host (biology); Gene; Bacteria; Biology; Gene expression; Gene cluster; Natural product; Genetics; Computational biology; Microbiology; Biochemistry; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001894304,0.0003217698,0.0002919832,0.0002498306,0.0002197729,0.000266162,0.0002368601,0.0002402225,0.000818997],"category_scores_gemma":[0.0001231013,0.000130139,0.0001760169,0.0002333244,0.0001361155,0.000176934,0.0004325808,0.0004974123,0.0005102713],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001846773,"about_ca_system_score_gemma":0.0002587849,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006069295,"about_ca_topic_score_gemma":0.0006962027,"domain_scores_codex":[0.9997957,0.00003910121,0.00002206683,0.00004252216,0.0000726205,0.00002805562],"domain_scores_gemma":[0.999927,0.00001311821,0.00002042481,0.00001236979,0.000006097418,0.00002104545],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003010199,0.00001419947,0.00008343175,0.0000185924,0.000001316837,0.00002380296,0.00001276245,0.00003718517,0.9988905,0.0001500198,0.00003124487,0.0007068708],"study_design_scores_gemma":[0.00001060684,0.0002153727,0.001448697,0.000008167467,0.00001146128,0.0003484011,0.00002762586,0.001487875,0.9878637,0.00007584665,0.00849021,0.00001208967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9362507,0.0008415726,0.05660137,0.0002008855,0.0001081809,0.0002092189,0.001882781,0.0007319649,0.003173272],"genre_scores_gemma":[0.9349719,0.0009915621,0.05438471,0.00007421622,0.00001810597,0.0001926602,0.003636732,0.0001358568,0.00559422],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000818997,"threshold_uncertainty_score":0.002739847,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0132898356550001,"score_gpt":0.2730405578687741,"score_spread":0.259750722213774,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}