{"id":"W2969238338","doi":"10.1186/s13059-019-1776-2","title":"Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation","year":2019,"lang":"en","type":"article","venue":"Genome biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":109,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Centre Hospitalier de l’Université de Montréal; McGill University Health Centre","funders":"National Institute of General Medical Sciences; National Heart, Lung, and Blood Institute; Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences; Biotechnology and Biological Sciences Research Council; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Medical Research Council; University of California, Davis; Directorate for Biological Sciences; National Institutes of Health; Akademie Věd České Republiky; Indiana Clinical and Translational Sciences Institute; Ministerstvo Školství, Mládeže a Tělovýchovy; Ministry of Education, Culture, Sports, Science and Technology; McGill University; McGill University Health Centre; National Cancer Institute; British Heart Foundation; National Center for Advancing Translational Sciences; Wellcome Trust; Japan Agency for Medical Research and Development; Canadian Institutes of Health Research; Royal Society; Wellcome","keywords":"CRISPR; Biology; Cas9; Conditional gene knockout; Genome editing; Genetics; Homologous recombination; Computational biology; Allele; Gene; Phenotype","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001427689,0.00009282714,0.0001832339,0.00004207827,0.00001542677,0.000001833125,0.00009454103,0.00013402,0.00005585564],"category_scores_gemma":[0.0003418393,0.00008935623,0.00009661983,0.00003402194,0.00004839988,0.000001906845,0.00004691912,0.00002978525,0.000001966766],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001289948,"about_ca_system_score_gemma":0.00005379718,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007206012,"about_ca_topic_score_gemma":0.000007483679,"domain_scores_codex":[0.9988467,0.0001352131,0.0003007522,0.0005455561,0.00004505732,0.0001267553],"domain_scores_gemma":[0.9989288,0.00002160179,0.0001151773,0.0006287096,0.000281687,0.00002404191],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004895407,0.00009511747,0.002050421,0.00004538471,0.00006164167,8.996444e-9,0.00004899007,0.001518231,0.9933602,0.00007406657,0.00005206497,0.002644907],"study_design_scores_gemma":[0.001389069,0.0004400472,0.004515103,0.000001912402,0.00002469497,0.000002287512,0.00002854867,0.006062658,0.98067,0.000101815,0.006656018,0.0001078131],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6073961,0.0008594191,0.3909902,0.0000215056,0.0001373678,0.000447134,0.0001195668,0.000002975345,0.00002570919],"genre_scores_gemma":[0.9381465,0.00004983581,0.0596907,0.00003647281,0.0001297509,0.00006197019,0.001770829,0.00001079802,0.0001031426],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3312995,"threshold_uncertainty_score":0.3643841,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06423970125261735,"score_gpt":0.4498523355932214,"score_spread":0.3856126343406041,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}