{"id":"W2969520863","doi":"10.1128/msphere.00408-19","title":"Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in <i>Leishmania</i>","year":2019,"lang":"en","type":"article","venue":"mSphere","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Government of Canada","keywords":"CRISPR; Biology; Genome editing; DNA repair; Cas9; DNA; Non-homologous end joining; Genetics; Gene; Guide RNA; Computational biology; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002915057,0.0002859004,0.0003552401,0.0001456338,0.0002607082,0.0005359381,0.0002510269,0.0004374178,0.001811599],"category_scores_gemma":[0.0003376329,0.0003037647,0.0003479696,0.0001271393,0.0002913264,0.0002957289,0.0003311154,0.0006664911,0.0009493461],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002824277,"about_ca_system_score_gemma":0.0003255491,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006968281,"about_ca_topic_score_gemma":0.001190776,"domain_scores_codex":[0.9996859,0.00004320245,0.00003308273,0.00009808297,0.0000776619,0.00006200001],"domain_scores_gemma":[0.999733,0.00006269164,0.00008081195,0.00004530927,0.00003538243,0.00004273275],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003437349,0.00000791525,0.0001731704,0.00005068928,0.000004689223,0.00005233909,0.00002090428,0.0000784662,0.9977672,0.0001674761,0.00004236205,0.0016005],"study_design_scores_gemma":[0.000001377338,0.00003517645,0.0008235162,0.0000044192,0.000004924791,0.0001300993,0.00001095982,0.0003912429,0.9965194,0.00003807473,0.002036197,0.000004619761],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9272969,0.002380155,0.06313206,0.0002364686,0.0000992336,0.00006548315,0.0004439735,0.0006948136,0.005650829],"genre_scores_gemma":[0.9825022,0.0005338922,0.01262799,0.00007369887,0.000006385228,0.00002597999,0.0004113722,0.0001329525,0.003685416],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001811599,"threshold_uncertainty_score":0.006060362,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006169463580340442,"score_gpt":0.2483449875310197,"score_spread":0.2421755239506793,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}