{"id":"W2969682794","doi":"10.1002/bit.27146","title":"Cell free biosynthesis of isoprenoids from isopentenol","year":2019,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Massachusetts Institute of Technology; U.S. Department of Energy","keywords":"Choline kinase; Biosynthesis; Biochemistry; Diterpene; Metabolic engineering; Metabolic pathway; Terpenoid; Enzyme; Synthetic biology; Chemistry; Mevalonate pathway; De novo synthesis; In vitro; Biology; Computational biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003581326,0.0005216167,0.0006343113,0.0002720397,0.0001852181,0.0006447521,0.000423751,0.0004372467,0.0005014607],"category_scores_gemma":[0.0003085068,0.0002096271,0.0005152895,0.0002704247,0.000307337,0.0005670773,0.0003994367,0.0008183725,0.0004085902],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004753814,"about_ca_system_score_gemma":0.0003043915,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006571985,"about_ca_topic_score_gemma":0.0009425491,"domain_scores_codex":[0.9997457,0.00006209431,0.00002825322,0.0000612591,0.0000806789,0.00002202962],"domain_scores_gemma":[0.9998232,0.00004635272,0.00003614481,0.00005017214,0.00002314516,0.0000210935],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000252122,0.00001140375,0.0001258882,0.00005838088,0.000009661657,0.000036573,0.00000942022,0.001132231,0.9968966,0.0004545163,0.00001807181,0.001221986],"study_design_scores_gemma":[0.000005723915,0.0000695395,0.0004613066,0.000006757637,0.00001771304,0.00006532911,0.000008887789,0.005001852,0.9910013,0.0002126219,0.003135555,0.00001340806],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.894656,0.004701816,0.09594359,0.0002014798,0.00009721368,0.00008359581,0.001507777,0.0003586543,0.002449741],"genre_scores_gemma":[0.9597452,0.002584146,0.03385741,0.00003857603,0.00001313307,0.00003895244,0.002003153,0.00005275339,0.00166661],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006571985,"threshold_uncertainty_score":0.003449142,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002640887615371881,"score_gpt":0.1568120320909019,"score_spread":0.15417114447553,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}