{"id":"W2969682794","doi":"10.1002/bit.27146","title":"Cell free biosynthesis of isoprenoids from isopentenol","year":2019,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; Massachusetts Institute of Technology; U.S. Department of Energy","keywords":"Choline kinase; Biosynthesis; Biochemistry; Diterpene; Metabolic engineering; Metabolic pathway; Terpenoid; Enzyme; Synthetic biology; Chemistry; Mevalonate pathway; De novo synthesis; In vitro; Biology; Computational biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006889875,0.0001804331,0.0002062658,0.00006969819,0.0000268614,0.000007484349,0.0003405552,0.0005423,0.00004278981],"category_scores_gemma":[0.00004079099,0.0001652622,0.00006437882,0.00009443965,0.0001017699,0.000003912726,0.0002588737,0.0001092708,0.0000129496],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004584516,"about_ca_system_score_gemma":0.00001379589,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002171849,"about_ca_topic_score_gemma":0.000002816927,"domain_scores_codex":[0.9991717,0.00001008178,0.0001805661,0.0003618266,0.00006495696,0.0002109039],"domain_scores_gemma":[0.9993329,0.00002100734,0.00006744336,0.0005157969,0.00001863929,0.00004417941],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004655985,0.00003449135,0.00208893,0.00004809039,0.00006445315,0.000001971873,0.00000529115,0.000005376203,0.9963546,0.00005405708,0.0002153704,0.001080842],"study_design_scores_gemma":[0.0003081182,0.0001323677,0.0007449131,0.0000324295,0.00002685466,0.00001105973,0.00005670306,0.00009843161,0.9917439,0.00002439185,0.006615961,0.0002048915],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958104,0.002651117,0.0003716638,0.0002920141,0.00009723748,0.0001045603,0.0001933202,0.00004563655,0.0004340133],"genre_scores_gemma":[0.9972144,0.000641457,0.00176739,0.0000373107,0.00005234854,0.000003987697,0.00005129134,0.00001524381,0.0002165936],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00640059,"threshold_uncertainty_score":0.6739198,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.002640887615371881,"score_gpt":0.1568120320909019,"score_spread":0.15417114447553,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}