{"id":"W2971742974","doi":"10.2144/btn-2019-0081","title":"Optimized Transformation, Overexpression and Purification of S100A10","year":2019,"lang":"en","type":"article","venue":"BioTechniques","topic":"S100 Proteins and Annexins","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University; Université du Québec à Trois-Rivières; Université Laval; Hôpital du Saint-Sacrement","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Circular dichroism; Transformation (genetics); Yield (engineering); Chemistry; Chromatography; Biophysics; Computational biology; Materials science; Biological system; Biochemistry; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008481406,0.0006890791,0.0006917801,0.0007620611,0.0004789259,0.0005900366,0.00072989,0.0006946679,0.001648552],"category_scores_gemma":[0.0005446736,0.0003936688,0.0005190508,0.0008240034,0.0003598841,0.0002873506,0.0007612616,0.001524038,0.002386446],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006688445,"about_ca_system_score_gemma":0.0005802916,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001387231,"about_ca_topic_score_gemma":0.00261001,"domain_scores_codex":[0.9991405,0.0001398358,0.0001267334,0.0001671787,0.0002869493,0.0001388408],"domain_scores_gemma":[0.9996887,0.00005983228,0.00005510043,0.00006260248,0.00007448805,0.00005929073],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004261114,0.00005821476,0.0001051519,0.00006761562,0.000007871879,0.0001047235,0.00003440884,0.0001421241,0.9971638,0.0002360278,0.0002950857,0.001742404],"study_design_scores_gemma":[0.0000232918,0.0001375966,0.001611975,0.00002156622,0.00002365421,0.0004366504,0.0000558282,0.001855956,0.9682675,0.0002185648,0.02732422,0.00002313296],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7731803,0.004248133,0.1861909,0.0008325274,0.0006686319,0.002850845,0.01943928,0.002575549,0.01001387],"genre_scores_gemma":[0.6606861,0.005295961,0.2478068,0.0002860335,0.00009200937,0.001808032,0.04821393,0.001464134,0.034347],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001648552,"threshold_uncertainty_score":0.00551492,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006764018811781547,"score_gpt":0.2423337512788236,"score_spread":0.2355697324670421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}