{"id":"W2971842044","doi":"10.1021/acssynbio.9b00232","title":"A Broad-Host-Range CRISPRi Toolkit for Silencing Gene Expression in <i>Burkholderia</i>","year":2019,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Cystic Fibrosis Canada; Institut national de la recherche scientifique; Cystic Fibrosis Foundation","keywords":"Gene silencing; Host (biology); Biology; Computational biology; Genetics; Gene expression; Burkholderia; Range (aeronautics); Gene; Burkholderia pseudomallei; Expression (computer science); Computer science; Bacteria; Engineering; Programming language","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004879154,0.000531101,0.0004170694,0.0004581923,0.0005820018,0.0007690441,0.000882632,0.0007444301,0.004443103],"category_scores_gemma":[0.0004558177,0.0005335453,0.0003740156,0.000326772,0.0003701056,0.0004424934,0.0009741241,0.00152975,0.003569415],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005724184,"about_ca_system_score_gemma":0.0005307715,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001281027,"about_ca_topic_score_gemma":0.004323298,"domain_scores_codex":[0.999479,0.00007138254,0.000041074,0.0001045024,0.0002236363,0.00008039935],"domain_scores_gemma":[0.9997843,0.00004139208,0.00004358059,0.00004987556,0.00003131807,0.00004949082],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000657397,0.00002862406,0.0001719169,0.0001192958,0.00001205895,0.0001106438,0.00004115019,0.0002203715,0.9826213,0.001374582,0.003925742,0.01130868],"study_design_scores_gemma":[0.0000225244,0.00009405972,0.0008836595,0.00003427068,0.00002291007,0.0005519402,0.00003891793,0.002483306,0.9350219,0.0006089257,0.06020524,0.00003225419],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3155162,0.007587885,0.5267697,0.003868524,0.001454895,0.001263174,0.02270392,0.05723319,0.06360251],"genre_scores_gemma":[0.7195172,0.003429442,0.2220214,0.001018381,0.00006313642,0.0006255241,0.01323021,0.002698051,0.0373967],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.004443103,"threshold_uncertainty_score":0.01486367,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008717419336382651,"score_gpt":0.2927382615832976,"score_spread":0.2840208422469149,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}