{"id":"W2971842044","doi":"10.1021/acssynbio.9b00232","title":"A Broad-Host-Range CRISPRi Toolkit for Silencing Gene Expression in <i>Burkholderia</i>","year":2019,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Cystic Fibrosis Canada; Institut national de la recherche scientifique; Cystic Fibrosis Foundation","keywords":"Gene silencing; Host (biology); Biology; Computational biology; Genetics; Gene expression; Burkholderia; Range (aeronautics); Gene; Burkholderia pseudomallei; Expression (computer science); Computer science; Bacteria; Engineering; Programming language","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002154472,0.0002119263,0.0002603078,0.00007399681,0.00003789107,0.00001270452,0.0002565913,0.0003204463,0.0000437963],"category_scores_gemma":[0.0001128547,0.000197118,0.00009926382,0.00007320622,0.00004702913,0.000003785133,0.0001386286,0.0001029501,0.00002887905],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001693911,"about_ca_system_score_gemma":0.00003061056,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002395749,"about_ca_topic_score_gemma":0.00001820706,"domain_scores_codex":[0.9986275,0.00005110601,0.0002818179,0.000544058,0.00005699833,0.0004385629],"domain_scores_gemma":[0.9992825,0.00005951848,0.0000603032,0.0004896251,0.00004392198,0.0000640989],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007196209,0.00003423172,0.004143051,0.00003728284,0.00001358006,0.000001009078,0.00007251499,0.0001617594,0.9936501,0.00005411194,0.000220477,0.001539919],"study_design_scores_gemma":[0.000848197,0.0003102881,0.001478049,0.00003891454,0.00001070752,0.00001844203,0.00008245911,0.000136732,0.9779968,0.0001053272,0.01869698,0.0002770901],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9772851,0.002163742,0.01881867,0.0001440879,0.000521131,0.0005267902,0.00003343827,0.00002278642,0.0004841811],"genre_scores_gemma":[0.9952356,0.0002602013,0.003560903,0.0002555544,0.0001886294,0.0001139972,0.00006031403,0.00003889122,0.0002859501],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0184765,"threshold_uncertainty_score":0.8038238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008717419336382651,"score_gpt":0.2927382615832976,"score_spread":0.2840208422469149,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}