{"id":"W2972870406","doi":"10.1128/aem.02566-19","title":"Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment","year":2020,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Biosensors and Analytical Detection","field":"Engineering","cited_by":53,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; Agriculture and Agri-Food Canada; University of British Columbia","funders":"Center for Produce Safety","keywords":"Viable but nonculturable; Salmonella enterica; Preharvest; Loop-mediated isothermal amplification; Microbiology; Propidium monoazide; Escherichia coli; Biology; Salmonella; Food microbiology; Foodborne pathogen; Bacteria; Food science; Postharvest; Listeria monocytogenes; Real-time polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000286789,0.0003656874,0.0002199462,0.0004514171,0.0001134025,0.0003757392,0.0002345091,0.0003993975,0.0004200165],"category_scores_gemma":[0.0003888291,0.00026074,0.0001439397,0.0002191318,0.000215649,0.0002863961,0.0002297646,0.0003655398,0.0002469384],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001446352,"about_ca_system_score_gemma":0.0001445045,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005180306,"about_ca_topic_score_gemma":0.001229638,"domain_scores_codex":[0.9997249,0.00003358355,0.00001211349,0.00006323298,0.0001398704,0.00002617141],"domain_scores_gemma":[0.9997992,0.00004592665,0.00005850191,0.0000142424,0.00005852502,0.00002364751],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002325082,0.00001463678,0.001315193,0.00002565785,0.000003131796,0.00002467679,0.00002774566,0.00005717632,0.9958663,0.00001527892,0.00001987091,0.002607097],"study_design_scores_gemma":[0.000003605854,0.0002243198,0.01474586,0.00001005592,0.00002148073,0.0001931062,0.0000987586,0.001843802,0.9822153,0.00003327156,0.0005989555,0.0000114992],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9653133,0.001077378,0.03197925,0.00007285759,0.0000258435,0.000060565,0.0003915399,0.0001740009,0.0009052819],"genre_scores_gemma":[0.959818,0.001056715,0.03519213,0.00004804999,0.00001219915,0.00008290244,0.0007335338,0.0000305148,0.003025787],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005180306,"threshold_uncertainty_score":0.0015167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004299027354430648,"score_gpt":0.1482595631972083,"score_spread":0.1439605358427776,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}