{"id":"W2973379642","doi":"10.1186/s40694-019-0076-7","title":"Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi","year":2019,"lang":"en","type":"article","venue":"Fungal Biology and Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":114,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Danmarks Tekniske Universitet; Concordia University","keywords":"CRISPR; Genome editing; Gene knockout; Cas9; Biology; Plasmid; Genetics; Gene; Transformation (genetics); Computational biology; Marker gene; Genome","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002167351,0.0001607477,0.0003217435,0.0003971867,0.00003623925,0.000003864745,0.0001658173,0.0005507698,0.00003337784],"category_scores_gemma":[0.00007322786,0.0001495657,0.0001181867,0.0003052854,0.00013831,0.000001162729,0.0001559079,0.0001230634,0.000001998985],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001038537,"about_ca_system_score_gemma":0.00001918486,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001845365,"about_ca_topic_score_gemma":0.00006602261,"domain_scores_codex":[0.9989144,0.00002624084,0.0002753295,0.0004378335,0.0000440167,0.0003021323],"domain_scores_gemma":[0.9994642,0.0000448546,0.00007947984,0.0003344645,0.00004652919,0.00003043155],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000122081,0.00003916754,0.04020664,0.00002621726,0.000332514,9.305382e-7,0.00001594715,0.003563421,0.9538723,0.0005089077,0.00004373168,0.001268113],"study_design_scores_gemma":[0.001669941,0.001026953,0.1756912,0.00001103189,0.000282758,0.00002557238,0.0003415752,0.01037992,0.8029757,0.0001228102,0.007063846,0.0004086562],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9763163,0.003208235,0.01949796,0.0001275423,0.0002549624,0.0002418266,0.0002425589,0.0000178749,0.00009277611],"genre_scores_gemma":[0.9975035,0.0002474054,0.001655603,0.00006733631,0.0000865055,0.00002952475,0.0003049734,0.00001073965,0.00009447533],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1508966,"threshold_uncertainty_score":0.6099112,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004916068669664316,"score_gpt":0.2464804574381338,"score_spread":0.2415643887684695,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}