{"id":"W2974023053","doi":"10.1128/aem.01644-19","title":"Using the Endogenous CRISPR-Cas System of <i>Heliobacterium modesticaldum</i> To Delete the Photochemical Reaction Center Core Subunit Gene","year":2019,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Basic Energy Sciences; U.S. Department of Energy","keywords":"CRISPR; Homologous recombination; Biology; Genetics; Plasmid; Gene; Site-specific recombination; Locus (genetics); In silico; Genome; Computational biology; Recombination; Recombinase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000748602,0.0001434138,0.0001411959,0.00001442898,0.00005956779,0.000005808388,0.0001367872,0.0001140968,0.000007139388],"category_scores_gemma":[0.000001769243,0.00009597546,0.00004485457,0.00002725435,0.0001146917,0.000001202787,0.0001735186,0.00008684318,0.00001158716],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002396141,"about_ca_system_score_gemma":0.000006104283,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001668118,"about_ca_topic_score_gemma":0.000001585212,"domain_scores_codex":[0.9993142,0.00001833078,0.0001677892,0.0002558594,0.00003223304,0.0002115478],"domain_scores_gemma":[0.9996085,0.0000148546,0.00004478492,0.0002840815,0.000004204723,0.00004359128],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006577757,0.00002307244,0.001104536,0.00001744987,0.00003403166,5.756986e-7,0.00006422659,0.000211166,0.9983246,0.00001613338,0.00002424347,0.000114174],"study_design_scores_gemma":[0.0003142217,0.00009184847,0.0008454457,0.000006250114,0.00002890624,0.0004309909,0.0003000052,0.0001210902,0.9952741,0.000002575911,0.002461707,0.0001228421],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966601,0.000222375,0.002377314,0.00002059069,0.00009557367,0.0004042349,0.00009173669,0.000005364334,0.0001227535],"genre_scores_gemma":[0.9990826,0.00006481679,0.000336813,0.0002394815,0.00006384407,0.00002061321,0.0001566932,0.00001831458,0.00001685621],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003050497,"threshold_uncertainty_score":0.3913766,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01226501226483877,"score_gpt":0.2292271934884061,"score_spread":0.2169621812235673,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}