{"id":"W2977570602","doi":"10.21769/bioprotoc.3075","title":"Ex vivo Culture and Lentiviral Transduction of Benign Prostatic Hyperplasia (BPH) Samples","year":2018,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Prostate Cancer Treatment and Research","field":"Medicine","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Medical Research Council; Prostate Cancer UK; Wellcome Trust","keywords":"Ex vivo; Prostate; Transduction (biophysics); Biology; Cell culture; Cancer research; Stroma; Immunohistochemistry; Hyperplasia; Cell growth; In vivo; Viral vector; Cell biology; Pathology; Molecular biology; Gene; Immunology; Medicine; Recombinant DNA; Cancer; Genetics; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001140446,0.0001446142,0.0002259123,0.00008294584,0.00009132962,0.00001959814,0.00005273852,0.00009084119,0.0003751189],"category_scores_gemma":[0.00002312483,0.00009504693,0.00005792008,0.0001692635,0.0003534366,0.0001011825,0.00002056691,0.00009984816,0.00001810684],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004547711,"about_ca_system_score_gemma":0.0001065801,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005312449,"about_ca_topic_score_gemma":0.00001818302,"domain_scores_codex":[0.9990031,0.00004056867,0.0002087816,0.0002597193,0.0002584204,0.0002294629],"domain_scores_gemma":[0.99949,0.00001410904,0.0000633442,0.0001852111,0.0001476105,0.00009978574],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00820506,0.001023654,0.06982363,0.003236173,0.0002867838,0.00003937675,0.005059345,1.453542e-7,0.853574,0.0007975672,0.01299874,0.04495554],"study_design_scores_gemma":[0.01036738,0.004848084,0.02028175,0.0003885253,0.00008895229,0.00006404142,0.0006100965,0.00003232544,0.7265047,0.000408393,0.2362021,0.0002036756],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.1437602,0.00004036377,0.00009405949,0.0005835594,0.00008313562,0.848137,0.00007992239,0.0000862089,0.007135558],"genre_scores_gemma":[0.2234255,0.00001031845,0.00173419,0.00005726677,0.000402489,0.7681919,0.0000286598,0.00003265089,0.00611708],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.2232033,"threshold_uncertainty_score":0.4107289,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04096952209950157,"score_gpt":0.3642650076793495,"score_spread":0.3232954855798479,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}