{"id":"W2978243962","doi":"10.1038/s41467-019-12448-3","title":"Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":139,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Canadian Institutes of Health Research; Ontario Genomics","keywords":"CRISPR; Plasmid; Bacterial conjugation; Salmonella enterica; Biology; Nuclease; Escherichia coli; Bacteria; Cas9; Computational biology; Genetics; Gene; Microbiology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004810998,0.0005047591,0.0004241651,0.0003924462,0.0002485417,0.0005792566,0.0003557675,0.0005737172,0.001335736],"category_scores_gemma":[0.0003503571,0.0002255591,0.0003579202,0.0002201082,0.0003848721,0.0003138809,0.0006492173,0.00104029,0.0007941728],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003918129,"about_ca_system_score_gemma":0.0004006881,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003001613,"about_ca_topic_score_gemma":0.0008417704,"domain_scores_codex":[0.9994469,0.0001017465,0.00005147675,0.0001245233,0.0001992636,0.00007613766],"domain_scores_gemma":[0.9997213,0.00006113411,0.00007094732,0.00007341979,0.00002918436,0.00004391846],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001324211,0.00001756686,0.00007815773,0.0000173002,0.000003775683,0.00002798329,0.000007560796,0.00005281169,0.9981714,0.0001551242,0.00004194272,0.001413068],"study_design_scores_gemma":[0.000002441524,0.00006123302,0.0004064887,0.000002180448,0.000004713114,0.0002151268,0.000007140935,0.0007245066,0.9971011,0.00004005497,0.001431544,0.000003348511],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8354447,0.001342138,0.1537249,0.0003832842,0.0001811979,0.0002417877,0.0005111723,0.001046197,0.007124529],"genre_scores_gemma":[0.952713,0.0006331234,0.04108927,0.0001071738,0.00001418797,0.00006123806,0.0005226251,0.0001356369,0.00472386],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001335736,"threshold_uncertainty_score":0.004468501,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009990487301903928,"score_gpt":0.2995847502046647,"score_spread":0.2895942629027607,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}