{"id":"W2978295770","doi":"10.3390/ijms20205212","title":"Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae","year":2019,"lang":"en","type":"article","venue":"International Journal of Molecular Sciences","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada; Advanced Research Projects Agency; Defense Advanced Research Projects Agency; U.S. Department of Defense","keywords":"Plasmid; Bacterial conjugation; Electroporation; DNA; Escherichia coli; Pilus; Saccharomyces cerevisiae; Exogenous DNA; Biology; Transformation (genetics); Chemistry; Cell biology; Genetics; Yeast; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006481798,0.0007628237,0.0005292186,0.0005785407,0.000317828,0.0005363058,0.0004104948,0.0003224798,0.001154703],"category_scores_gemma":[0.0005446977,0.0002983988,0.0004410868,0.0005030312,0.0003107562,0.0002527931,0.0006986468,0.0008653566,0.001041263],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002819695,"about_ca_system_score_gemma":0.0004325793,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001009425,"about_ca_topic_score_gemma":0.002213831,"domain_scores_codex":[0.999278,0.0001482032,0.0001578518,0.0001336108,0.0002169077,0.0000653937],"domain_scores_gemma":[0.9996308,0.00007103628,0.00008420608,0.00009877207,0.00006701085,0.00004809747],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003621252,0.00002436439,0.0001118521,0.00005713378,0.000005425321,0.00007030122,0.00002418477,0.00008410722,0.9968293,0.0001410317,0.00005027289,0.002565668],"study_design_scores_gemma":[0.000006322924,0.00009550878,0.0006457714,0.000009028247,0.00001457618,0.0001260641,0.00001854769,0.0005404453,0.9941294,0.00005025122,0.004355472,0.000008669043],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7707378,0.001447444,0.2164867,0.0002753534,0.0003154278,0.001255464,0.002261921,0.001608921,0.005610951],"genre_scores_gemma":[0.789046,0.00249497,0.1863933,0.0001440478,0.00002867163,0.0006720162,0.007813733,0.0006423024,0.01276497],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001154703,"threshold_uncertainty_score":0.003862798,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01025592175741414,"score_gpt":0.3069265214931456,"score_spread":0.2966705997357315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}