{"id":"W2980026322","doi":"10.1126/sciadv.aaw8451","title":"Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method","year":2019,"lang":"en","type":"article","venue":"Science Advances","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":108,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"National Natural Science Foundation of China","keywords":"Fucosyltransferase; Throughput; Computer science; High-throughput screening; Computational biology; Directed evolution; Biology; Genetics; Telecommunications; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006085138,0.0001138642,0.0001326216,0.0001387522,0.0001524138,0.00003371072,0.0003368579,0.00006044551,0.00004091649],"category_scores_gemma":[0.0001162714,0.0001039825,0.00004922261,0.0006680312,0.0002725373,0.00008444644,0.00005362086,0.00007753497,0.000005793196],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003453487,"about_ca_system_score_gemma":0.0002049453,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001142527,"about_ca_topic_score_gemma":0.00004485686,"domain_scores_codex":[0.9984634,0.00009319312,0.0001928121,0.0004846653,0.0004363645,0.0003295784],"domain_scores_gemma":[0.9991833,0.00001872482,0.00007968217,0.0003465147,0.0002640135,0.0001077691],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006807332,0.00007050519,0.001302513,0.00002112629,0.000002836217,7.939041e-7,0.00004043338,0.004283304,0.9842792,0.00003817172,0.000001319281,0.009891679],"study_design_scores_gemma":[0.0004180698,0.0004233386,0.0009803361,0.00002087205,0.00000430722,0.00001008389,0.0001998358,0.007384196,0.9887541,0.00007955854,0.001583664,0.0001416161],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8777667,0.0006134597,0.120323,0.0000174641,0.0001037382,0.0002346252,0.00001084683,0.0000188517,0.0009113365],"genre_scores_gemma":[0.9037471,0.00001359778,0.09598275,0.00002148212,0.00004212516,0.000004051657,0.00001347925,0.000009673271,0.0001657342],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02598042,"threshold_uncertainty_score":0.4240282,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01893083318517807,"score_gpt":0.3263128314693063,"score_spread":0.3073819982841283,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}