{"id":"W2980026322","doi":"10.1126/sciadv.aaw8451","title":"Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method","year":2019,"lang":"en","type":"article","venue":"Science Advances","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":108,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"National Natural Science Foundation of China","keywords":"Fucosyltransferase; Throughput; Computer science; High-throughput screening; Computational biology; Directed evolution; Biology; Genetics; Telecommunications; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003768525,0.0005774574,0.0003683388,0.0003420341,0.0001593474,0.0005085808,0.0006094545,0.0003924641,0.00080204],"category_scores_gemma":[0.0002223831,0.0002046712,0.0003838099,0.0004572994,0.0001665392,0.0001646571,0.0005096986,0.0007422952,0.0005734173],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003071346,"about_ca_system_score_gemma":0.0002562621,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001127543,"about_ca_topic_score_gemma":0.001585462,"domain_scores_codex":[0.9997503,0.00005161066,0.0000224635,0.00004552523,0.00009186778,0.00003822708],"domain_scores_gemma":[0.9998797,0.00002799511,0.00003403331,0.0000191206,0.00001585791,0.0000231975],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004000909,0.00004412822,0.0001583716,0.00001892946,0.000008754459,0.00005141984,0.00001147699,0.0002374369,0.9969292,0.0001222985,0.00005398758,0.002323959],"study_design_scores_gemma":[0.00001489912,0.0001601149,0.001365774,0.000003188822,0.0000253417,0.0002248228,0.0000194044,0.006075191,0.9897647,0.00003865018,0.002299318,0.000008725953],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9238473,0.000532043,0.07073975,0.0001744052,0.00007361408,0.0002666195,0.001521082,0.0005386422,0.002306713],"genre_scores_gemma":[0.931022,0.0005549614,0.0606516,0.00006027949,0.000007272887,0.0001431723,0.002298439,0.00009965798,0.005162495],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001127543,"threshold_uncertainty_score":0.002683103,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01893083318517807,"score_gpt":0.3263128314693063,"score_spread":0.3073819982841283,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}