{"id":"W2980844478","doi":"10.1002/jctb.6250","title":"Recombinant expression of <i>Aspergillus niger</i> GH10 endo‐xylanase in <i>Pichia pastoris</i> by constructing a double‐plasmid co‐expression system","year":2019,"lang":"en","type":"article","venue":"Journal of Chemical Technology & Biotechnology","topic":"Biofuel production and bioconversion","field":"Engineering","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lakehead University","funders":"Chinese Academy of Tropical Agricultural Sciences; Six Talent Peaks Project in Jiangsu Province; Natural Science Foundation of Jiangsu Province; National Natural Science Foundation of China","keywords":"Pichia pastoris; Xylanase; Aspergillus niger; Plasmid; Expression vector; Heterologous expression; Recombinant DNA; Heterologous; Biochemistry; Biology; Enzyme; Chemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002329337,0.0008003711,0.0004826238,0.0002609641,0.0001697176,0.0004783946,0.0003994236,0.0002831762,0.0004909905],"category_scores_gemma":[0.00013347,0.0003257587,0.0004290213,0.0003934465,0.0001838843,0.0002887008,0.00029529,0.0007307812,0.0005591526],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004901902,"about_ca_system_score_gemma":0.0002601314,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00121341,"about_ca_topic_score_gemma":0.001201742,"domain_scores_codex":[0.9997564,0.00003585155,0.00004429027,0.00005680728,0.00007168687,0.00003494812],"domain_scores_gemma":[0.9999175,0.00001472799,0.00002188247,0.00001618519,0.00001381498,0.00001581571],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000205886,0.00001503299,0.00008021261,0.00004355959,0.000006532315,0.00004108554,0.000008343253,0.00009804254,0.9987908,0.00006669166,0.00004622853,0.0007829074],"study_design_scores_gemma":[0.00001010406,0.0000715772,0.001102173,0.000007255217,0.00003048559,0.0002305396,0.00001572102,0.001891068,0.9929715,0.00003076471,0.003628923,0.000009868496],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9363868,0.001460601,0.05467736,0.000282515,0.0001480969,0.0002326592,0.002373473,0.0007966394,0.003641831],"genre_scores_gemma":[0.9288253,0.001611518,0.05586334,0.00009966987,0.00002971545,0.0001628952,0.005873847,0.0001706104,0.00736318],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00121341,"threshold_uncertainty_score":0.003556609,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003899985331569735,"score_gpt":0.1919117403175832,"score_spread":0.1880117549860135,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}