{"id":"W2982038705","doi":"10.1038/s41467-019-12829-8","title":"Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Genomics; McGill University","funders":"Japan Agency for Medical Research and Development; Compute Canada; Genome Canada","keywords":"Genome editing; Biology; Genetics; Computational biology; Gene; Homologous recombination; Cas9; CRISPR; Nuclease; DNA repair; Human genome; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001849788,0.0001091368,0.0001308421,0.00005593838,0.0000936135,0.00001240193,0.0008384806,0.0002940256,0.00001844932],"category_scores_gemma":[0.0005753693,0.0001022878,0.00007780018,0.0001288382,0.0001128011,0.000004758896,0.0004805221,0.0002962917,0.00001145608],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001373273,"about_ca_system_score_gemma":0.00003678512,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007586608,"about_ca_topic_score_gemma":0.00005967153,"domain_scores_codex":[0.9992748,0.00005797784,0.0002416898,0.0001926243,0.0000743717,0.0001585552],"domain_scores_gemma":[0.9980777,0.0001168204,0.0001048604,0.001490318,0.0001774476,0.00003289227],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001349492,0.00005916192,0.01382902,0.00002221192,0.00004479035,2.698224e-7,0.0000625553,0.001209647,0.982933,0.0004665418,0.0009952914,0.0003639818],"study_design_scores_gemma":[0.001219787,0.0004896121,0.1319804,0.00008674799,0.00008487781,0.00003724199,0.0007148345,0.0005479425,0.6153041,0.001961939,0.2469571,0.0006155428],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9762644,0.01239309,0.004813429,0.001073734,0.0002219606,0.0004162708,0.00008899669,0.00004691435,0.00468119],"genre_scores_gemma":[0.9769452,0.001143671,0.02092853,0.00007554604,0.00005026273,0.00002928501,0.0005718184,0.00001390525,0.0002417827],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.367629,"threshold_uncertainty_score":0.4171177,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009843258708605812,"score_gpt":0.2900856849351077,"score_spread":0.2802424262265019,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}