{"id":"W2982525250","doi":"10.1038/s41467-019-12902-2","title":"Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"CIHR Skin Research Training Centre; Instituto de Salud Carlos III; Canadian Institutes of Health Research; Generalitat de Catalunya; Diabetes Canada; Government of Canada; Centres de Recerca de Catalunya; European Foundation for the Study of Diabetes; Eli Lilly and Company","keywords":"Avidity; Major histocompatibility complex; Flow cytometry; T-cell receptor; Peptide; Antigen; Chemistry; Reprogramming; Computational biology; Cell biology; Recombinant DNA; Fusion protein; Biology; Biochemistry; Molecular biology; T cell; Cell; Immune system; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003086054,0.0002972551,0.0001456912,0.0001485832,0.0001063316,0.0004549788,0.0001933141,0.0002744622,0.001382258],"category_scores_gemma":[0.0003204864,0.00009898042,0.0001067769,0.0001545655,0.0001892421,0.0002692213,0.0002408269,0.0004203381,0.0004896884],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002628426,"about_ca_system_score_gemma":0.00009857106,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000199669,"about_ca_topic_score_gemma":0.0004807253,"domain_scores_codex":[0.9997358,0.00006894075,0.0000174973,0.00006433539,0.00006855537,0.00004490452],"domain_scores_gemma":[0.9998197,0.00005429484,0.00004705374,0.0000258015,0.00002331398,0.00002984333],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002486172,0.0000080821,0.00004719189,0.00002609046,0.000002346277,0.0000192681,0.00001575167,0.00008137061,0.9982297,0.0001720436,0.00003237811,0.001340888],"study_design_scores_gemma":[0.000002586067,0.00005870881,0.0002583649,0.000003162556,0.000005107493,0.00009142405,0.00000842472,0.0002926415,0.9974505,0.0000349195,0.001792442,0.000001707399],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9588377,0.002330515,0.0342175,0.0002228059,0.00005682171,0.00004123358,0.0002939972,0.0002511569,0.003748275],"genre_scores_gemma":[0.9844218,0.000632544,0.01135582,0.00007001596,0.00001507547,0.00002165484,0.0003858942,0.0000594466,0.003037743],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001382258,"threshold_uncertainty_score":0.004624128,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03223781693347265,"score_gpt":0.3365893867078579,"score_spread":0.3043515697743852,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}