{"id":"W2988404531","doi":"10.1007/s11033-019-05176-w","title":"A simplified quick microbial genomic DNA extraction via freeze–thawing cycles","year":2019,"lang":"en","type":"article","venue":"Molecular Biology Reports","topic":"Legume Nitrogen Fixing Symbiosis","field":"Agricultural and Biological Sciences","cited_by":27,"is_retracted":false,"has_abstract":false,"ca_institutions":"Lakehead University","funders":"Jiangsu Agricultural Science and Technology Independent Innovation Fund; Priority Academic Program Development of Jiangsu Higher Education Institutions","keywords":"genomic DNA; DNA extraction; DNA; Biology; Extraction (chemistry); Chromatography; Bacillus pumilus; Nucleic acid; Biochemistry; Chemistry; Polymerase chain reaction; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001120043,0.00228845,0.001367728,0.001829799,0.001189351,0.0006646883,0.002017865,0.0009540157,0.01313361],"category_scores_gemma":[0.001406379,0.0009515039,0.001059321,0.001153762,0.0009589298,0.000562741,0.001650102,0.002159724,0.00974436],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003176687,"about_ca_system_score_gemma":0.001283885,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001625925,"about_ca_topic_score_gemma":0.006360863,"domain_scores_codex":[0.9985594,0.0001906954,0.0002018115,0.0004390896,0.000408474,0.0002006359],"domain_scores_gemma":[0.9992419,0.0001956728,0.0000592851,0.0002473199,0.000196185,0.00005953308],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001458244,0.0001938616,0.0008073224,0.0003209102,0.00004348701,0.0002500804,0.0001130403,0.0002578439,0.9749111,0.0003916665,0.002491824,0.02007309],"study_design_scores_gemma":[0.0002929513,0.002221276,0.0225769,0.0003159639,0.0005773699,0.001432122,0.0002047499,0.003838803,0.7474824,0.002493794,0.2183849,0.0001786912],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1133538,0.003778452,0.8341347,0.0006562034,0.001795454,0.01120402,0.01326434,0.006514865,0.01529817],"genre_scores_gemma":[0.08415265,0.003814056,0.834493,0.001554064,0.0005488821,0.01806168,0.03383687,0.000921197,0.02261753],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01313361,"threshold_uncertainty_score":0.04393631,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007583316894545232,"score_gpt":0.2290418987959293,"score_spread":0.221458581901384,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}