{"id":"W2988404531","doi":"10.1007/s11033-019-05176-w","title":"A simplified quick microbial genomic DNA extraction via freeze–thawing cycles","year":2019,"lang":"en","type":"article","venue":"Molecular Biology Reports","topic":"Legume Nitrogen Fixing Symbiosis","field":"Agricultural and Biological Sciences","cited_by":27,"is_retracted":false,"has_abstract":false,"ca_institutions":"Lakehead University","funders":"Jiangsu Agricultural Science and Technology Independent Innovation Fund; Priority Academic Program Development of Jiangsu Higher Education Institutions","keywords":"genomic DNA; DNA extraction; DNA; Biology; Extraction (chemistry); Chromatography; Bacillus pumilus; Nucleic acid; Biochemistry; Chemistry; Polymerase chain reaction; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002942059,0.0002223875,0.0002829091,0.00002585621,0.0001263104,0.00003915751,0.0001865333,0.0003084906,0.0004328769],"category_scores_gemma":[0.00004812317,0.0001059215,0.0002116367,0.0001708868,0.00006779487,0.00007285302,0.0001251878,0.0001789926,0.0002159917],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000581182,"about_ca_system_score_gemma":0.00001594268,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000635936,"about_ca_topic_score_gemma":0.0001065153,"domain_scores_codex":[0.9982458,0.000132298,0.0004179134,0.0006588272,0.000106565,0.0004385464],"domain_scores_gemma":[0.9993027,0.00006217604,0.0002882988,0.0001936588,0.00005866949,0.00009446767],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001435579,0.00004094664,0.06386377,0.000003935539,0.00003924439,0.0001360224,0.00001538066,9.199357e-7,0.9320363,0.00002629838,0.00009109455,0.003731781],"study_design_scores_gemma":[0.0001291451,0.0001860527,0.1041997,0.00001141937,0.00003199045,0.0009143916,0.00002591492,0.000006170128,0.8886403,0.00157782,0.003907939,0.0003691779],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973953,0.0001981585,0.00004771277,0.0004519392,0.000824573,0.0003492713,0.00001132,0.0001388815,0.0005827867],"genre_scores_gemma":[0.9985926,0.000002861722,0.0002821072,0.0005252803,0.0001701318,0.000016158,0.0001778542,0.000003873218,0.0002291373],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.043396,"threshold_uncertainty_score":0.4739698,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007583316894545232,"score_gpt":0.2290418987959293,"score_spread":0.221458581901384,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}