{"id":"W2989392176","doi":"10.1007/s10529-019-02749-4","title":"Identification and characterization of sequence signatures in the Bacillus subtilis promoter Pylb for tuning promoter strength","year":2019,"lang":"en","type":"article","venue":"Biotechnology Letters","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute","funders":"National Natural Science Foundation of China","keywords":"Promoter; Gene; Biology; Bacillus subtilis; Molecular biology; Mutant; Consensus sequence; Nucleic acid sequence; Genetics; Gene expression; Peptide sequence; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003203749,0.0003363501,0.0003527277,0.000362709,0.0001329651,0.0002835734,0.0001788857,0.0003723183,0.0005679116],"category_scores_gemma":[0.00068981,0.000261891,0.0002856162,0.0002823618,0.0002308881,0.0002155232,0.000254515,0.0005320173,0.0004376516],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002334666,"about_ca_system_score_gemma":0.000448635,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005193296,"about_ca_topic_score_gemma":0.00074149,"domain_scores_codex":[0.999666,0.00005056618,0.00002295588,0.00006943951,0.0001522772,0.00003874959],"domain_scores_gemma":[0.9994743,0.00009926243,0.0001953461,0.00003879459,0.00007715917,0.0001149974],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004919349,0.00001307217,0.0003333457,0.00002349538,0.000001590724,0.00001292976,0.000007622169,0.00008123548,0.9981517,0.00001761009,0.000007756531,0.001300505],"study_design_scores_gemma":[0.00001537956,0.0001515024,0.005354991,0.00001080961,0.0000136905,0.0002396736,0.000021312,0.002187805,0.9912009,0.0000372863,0.0007557925,0.00001094809],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9706193,0.0007571725,0.02684799,0.0001614346,0.00001507089,0.00007186003,0.0007153702,0.0001889328,0.0006229424],"genre_scores_gemma":[0.9630236,0.0004166524,0.03363381,0.00006251101,0.000009454435,0.00009592177,0.0018782,0.00009845098,0.0007814931],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005679116,"threshold_uncertainty_score":0.001899838,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006344426189842571,"score_gpt":0.2083473789345806,"score_spread":0.202002952744738,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}