{"id":"W2989940176","doi":"10.1016/j.pep.2019.105541","title":"The impact of a His-tag on DNA binding by RNA polymerase alpha-C-terminal domain from Helicobacter pylori","year":2019,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"CTD; DNA; Protein subunit; Biology; Biochemistry; Molecular biology; Computational biology; Chemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002366928,0.0001606696,0.00013078,0.00003154571,0.0001313273,0.00004599342,0.0001814967,0.0001513134,0.00009211446],"category_scores_gemma":[0.00003582631,0.0001028808,0.00008739629,0.00004369062,0.00004159408,0.00001012724,0.00004105129,0.00007563958,0.00002537531],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001271376,"about_ca_system_score_gemma":0.00002911897,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004798482,"about_ca_topic_score_gemma":6.358136e-7,"domain_scores_codex":[0.9989627,0.0001575991,0.0002213502,0.0003256778,0.0001564293,0.0001762319],"domain_scores_gemma":[0.9992077,0.00003264591,0.0002143571,0.0004339237,0.00003300926,0.0000783963],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004122485,0.00005151501,0.000172528,0.000008218771,0.00002043726,3.003644e-7,0.00006390367,3.80441e-7,0.9813874,0.00006050553,0.0003905301,0.01743202],"study_design_scores_gemma":[0.0004210331,0.0003276853,0.0004844112,0.00008655745,0.000004732048,0.000001427467,0.00005566076,0.00001324106,0.9933028,0.0002846254,0.004882034,0.0001357712],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973426,0.0009593832,0.0004438238,0.0001758462,0.00006478075,0.0005935321,0.0000753067,0.000008582784,0.0003361524],"genre_scores_gemma":[0.998027,0.0001631958,0.0003878384,0.00002799384,0.00009202159,0.00011969,0.000137626,0.00001926985,0.001025368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01729625,"threshold_uncertainty_score":0.4195358,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00816062371519004,"score_gpt":0.2476878021070749,"score_spread":0.2395271783918848,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}