{"id":"W2989974442","doi":"10.3389/fmicb.2019.02686","title":"Patterns of PCR Amplification Artifacts of the Fungal Barcode Marker in a Hybrid Mushroom","year":2019,"lang":"en","type":"article","venue":"Frontiers in Microbiology","topic":"Fungal Biology and Applications","field":"Medicine","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; Polymerase chain reaction; Genetics; Internal transcribed spacer; genomic DNA; Gene; DNA; Polymerase; Mushroom; Molecular biology; Ribosomal RNA; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005968516,0.0005813733,0.0004438309,0.0009320017,0.0002065471,0.0004879643,0.000351383,0.0005922994,0.0008498499],"category_scores_gemma":[0.001633253,0.0003596698,0.0005054765,0.0006555692,0.0004966413,0.0002183108,0.0003836901,0.0007663922,0.00060908],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001885523,"about_ca_system_score_gemma":0.0001586376,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006463872,"about_ca_topic_score_gemma":0.0006729747,"domain_scores_codex":[0.9984463,0.0001957654,0.000222337,0.0005580733,0.0004670053,0.000110481],"domain_scores_gemma":[0.9962403,0.001364607,0.001282118,0.0003872329,0.000445441,0.0002803123],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009994975,0.00002060508,0.00182788,0.00005460544,0.00001727632,0.0001708117,0.00007713485,0.00005582828,0.9960065,0.0000236816,0.00001262058,0.001633025],"study_design_scores_gemma":[0.000006301038,0.0002848778,0.02874815,0.00002255224,0.00009138221,0.001168616,0.0001415665,0.001012788,0.9674751,0.00006569277,0.0009670557,0.00001589379],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9819847,0.001068706,0.01548637,0.00006395559,0.00002943025,0.0000387498,0.00025105,0.0002013968,0.0008757291],"genre_scores_gemma":[0.9794762,0.0004644899,0.01747374,0.00009830376,0.00001214712,0.00005756702,0.0008197533,0.0001050824,0.001492822],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009320017,"threshold_uncertainty_score":0.003156483,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007237787760220173,"score_gpt":0.2221069325347786,"score_spread":0.2148691447745585,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}