{"id":"W2990149922","doi":"10.3791/60422","title":"In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated &lt;em&gt;Caenorhabditis elegans&lt;/em&gt; Embryo Blastomeres and Adhesive Polystyrene Beads","year":2019,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Multicellular organism; Blastomere; Cell biology; Caenorhabditis elegans; Cell; Cell fate determination; Biology; Embryo; Cell adhesion; In vitro; Cell type; Cell division; Genetics; Embryogenesis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002263194,0.0002897213,0.0005505974,0.0001977342,0.00004817475,0.0000456847,0.000214424,0.0002104055,0.00009319514],"category_scores_gemma":[0.00002316249,0.0002588388,0.0001232452,0.00008304983,0.00007365101,0.00003383381,0.00007896519,0.0001704485,0.000005197375],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006592933,"about_ca_system_score_gemma":0.0001584866,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001503722,"about_ca_topic_score_gemma":0.00009829196,"domain_scores_codex":[0.9981826,0.0001527123,0.0006802777,0.0003195309,0.0003495294,0.0003153264],"domain_scores_gemma":[0.9986601,0.00001924703,0.0006802753,0.0002518582,0.0002344306,0.0001540888],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001877998,0.000341503,0.005187138,0.00004920517,0.0001705751,0.00003090487,0.001271568,0.0000398158,0.9906012,0.000006582141,0.00009527825,0.0003282397],"study_design_scores_gemma":[0.005498729,0.002725588,0.004695471,0.0001285169,0.00006915122,0.0001187876,0.0006104875,0.0001476315,0.9852784,0.00000640559,0.0004284589,0.0002924113],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9932978,0.001721175,0.004142357,0.0000224703,0.000390091,0.0002753192,0.00004016352,0.000004578276,0.0001060124],"genre_scores_gemma":[0.9982487,0.0004043894,0.0008113876,0.0001352367,0.0001585896,0.000005157327,0.00004132835,0.00003717003,0.0001580581],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00532283,"threshold_uncertainty_score":0.9999864,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007250324041776227,"score_gpt":0.2820060924721767,"score_spread":0.2747557684304004,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}