{"id":"W2991193264","doi":"10.1038/s41467-019-13403-y","title":"CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci","year":2019,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lunenfeld-Tanenbaum Research Institute; University of Toronto; Mount Sinai Hospital","funders":"","keywords":"CRISPR; Genome editing; Cas9; Subgenomic mRNA; Guide RNA; Biology; Computational biology; Homologous recombination; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000173307,0.00008972448,0.0001059759,0.00002985877,0.00007805684,0.00001217942,0.000390412,0.0002042555,0.000008620077],"category_scores_gemma":[0.0001014713,0.00009737414,0.00007807491,0.00007693468,0.0000343965,0.000005943798,0.0001549297,0.0002144649,0.00000157251],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001452768,"about_ca_system_score_gemma":0.00002627274,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000148324,"about_ca_topic_score_gemma":0.00006483734,"domain_scores_codex":[0.9994714,0.00003720693,0.0001380765,0.0001604877,0.00007374246,0.0001191342],"domain_scores_gemma":[0.9988327,0.00004867753,0.00009349338,0.0008244555,0.0001731099,0.00002755016],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001348921,0.00005825825,0.000824039,0.00002670513,0.00003506207,1.300975e-8,0.00003121903,0.0001264959,0.9880726,0.0007203176,0.007535505,0.002556236],"study_design_scores_gemma":[0.0005973025,0.00007445562,0.00129429,0.00001731827,0.00002577963,0.000002015223,0.00004489938,0.00262541,0.9378746,0.0001928564,0.05711734,0.0001337477],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9674654,0.006083159,0.02177516,0.001612599,0.0004331828,0.0006144654,0.0001368653,0.00003050465,0.001848642],"genre_scores_gemma":[0.9933947,0.0001924948,0.005280536,0.00004626353,0.00008353362,0.00002930198,0.0006145984,0.00001750017,0.000341112],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05019808,"threshold_uncertainty_score":0.3970802,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01015169466143732,"score_gpt":0.3447118946815685,"score_spread":0.3345602000201311,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}