{"id":"W2994447848","doi":"10.1182/blood.v106.11.2382.2382","title":"A Simple Two-Step Method for the Isolation of Human CD4+CD25+bright / FOXP3+ Regulatory T Cells Directly from Whole Blood.","year":2005,"lang":"en","type":"article","venue":"Blood","topic":"T-cell and B-cell Immunology","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Terry Fox Research Institute; Vancouver Coastal Health Research Institute; Vancouver Coastal Health; Stemcell Technologies","funders":"","keywords":"Ficoll; IL-2 receptor; Cell sorting; Immunomagnetic separation; Biology; Molecular biology; FOXP3; Centrifugation; T cell; Differential centrifugation; Antibody; Peripheral blood mononuclear cell; Cell biology; Immune system; Immunology; Flow cytometry; In vitro; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004563096,0.001098972,0.0005495769,0.0008418598,0.0004591748,0.0003297865,0.00089053,0.0004448004,0.007146801],"category_scores_gemma":[0.0003345869,0.0003824469,0.0005837771,0.0004258968,0.0002244686,0.0001998648,0.0006754237,0.001050584,0.008152758],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001504523,"about_ca_system_score_gemma":0.0004466693,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004674657,"about_ca_topic_score_gemma":0.001423201,"domain_scores_codex":[0.9995182,0.00007989488,0.0000512761,0.0001072578,0.0001725853,0.00007098534],"domain_scores_gemma":[0.9998457,0.00004359757,0.00001555566,0.00003052994,0.00003173709,0.00003280887],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001044088,0.0001372555,0.0002348649,0.0002148986,0.00002490319,0.0001472515,0.00004464229,0.00007898292,0.9791852,0.0002127726,0.001770894,0.01784405],"study_design_scores_gemma":[0.0003575471,0.001389814,0.0127737,0.0001296733,0.0003274282,0.002892567,0.00006172792,0.003623869,0.8753092,0.0007585101,0.1022792,0.00009669661],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1296439,0.01284916,0.8127354,0.0008641626,0.001691133,0.01140904,0.007514208,0.004146144,0.01914685],"genre_scores_gemma":[0.2416287,0.005046075,0.692979,0.001199155,0.0004080676,0.01170383,0.02055892,0.0005729723,0.02590328],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.007146801,"threshold_uncertainty_score":0.02390844,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01115015567577649,"score_gpt":0.2521791336102049,"score_spread":0.2410289779344284,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}