{"id":"W2995327781","doi":"10.21769/bioprotoc.3455","title":"Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells","year":2019,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Autophagy in Disease and Therapy","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Autophagy; Autophagosome; Cell biology; Organelle; Lysosome; Green fluorescent protein; mCherry; Biology; ATG8; Chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0007623686,0.0003646153,0.0005412066,0.0001565615,0.0001800809,0.00005157712,0.000161449,0.0002757818,0.0006442454],"category_scores_gemma":[0.0001061896,0.0003125576,0.0003044979,0.0002919483,0.00006815283,0.0001387057,0.00005634323,0.0002628147,0.0004702002],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002067117,"about_ca_system_score_gemma":0.0003127244,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000456952,"about_ca_topic_score_gemma":0.000002413637,"domain_scores_codex":[0.9976497,0.0001638092,0.0004991948,0.0006400084,0.0003771203,0.0006702006],"domain_scores_gemma":[0.9984373,0.0001846818,0.0002736561,0.0005490538,0.0002723635,0.0002829783],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.004647424,0.0006715257,0.00429978,0.001791955,0.0004962231,0.0001890135,0.0006026762,0.00002029068,0.9085534,0.0007251071,0.0370656,0.04093706],"study_design_scores_gemma":[0.01010578,0.001172307,0.00428575,0.0002544549,0.00009402942,0.00002820302,0.0001316059,0.003170699,0.7351638,0.0008831297,0.2441719,0.0005383359],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.02635119,0.00001828295,0.001784635,0.0004412122,0.0003823884,0.9624693,0.00005128618,0.0004194983,0.008082221],"genre_scores_gemma":[0.160028,6.592578e-7,0.01020257,0.001734642,0.001460048,0.8171347,0.00006879224,0.0001885167,0.009182078],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.2071063,"threshold_uncertainty_score":0.9999326,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01547713445817227,"score_gpt":0.3118249865332434,"score_spread":0.2963478520750711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}