{"id":"W2995327781","doi":"10.21769/bioprotoc.3455","title":"Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells","year":2019,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Autophagy in Disease and Therapy","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Autophagy; Autophagosome; Cell biology; Organelle; Lysosome; Green fluorescent protein; mCherry; Biology; ATG8; Chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001611287,0.001995505,0.001208351,0.001631673,0.0008688858,0.0007768665,0.002097812,0.001420561,0.00526235],"category_scores_gemma":[0.0006919941,0.0008138128,0.001034694,0.001154774,0.0005074288,0.0009731566,0.0009839995,0.002364679,0.006458767],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008256882,"about_ca_system_score_gemma":0.001045232,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005230291,"about_ca_topic_score_gemma":0.001299931,"domain_scores_codex":[0.9977174,0.0004804995,0.0002860107,0.0005824619,0.0007780815,0.0001555432],"domain_scores_gemma":[0.999346,0.0001506857,0.0001187255,0.0001284264,0.0001883022,0.0000678527],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008913556,0.00005850048,0.0001480472,0.0002788882,0.00001546343,0.00006296779,0.00006417341,0.0001185455,0.9909967,0.00066767,0.001344525,0.006155215],"study_design_scores_gemma":[0.00001685009,0.0001626263,0.0007854603,0.00003953397,0.00003141756,0.000362592,0.00002039445,0.001529541,0.9826481,0.0002266707,0.01414471,0.00003213268],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07488266,0.006742686,0.8785433,0.0005320882,0.0005394354,0.002235354,0.00528888,0.01277563,0.01846],"genre_scores_gemma":[0.1098553,0.008800618,0.814328,0.0007906813,0.000113019,0.008163184,0.01648966,0.001328346,0.04013121],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00526235,"threshold_uncertainty_score":0.01760429,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01547713445817227,"score_gpt":0.3118249865332434,"score_spread":0.2963478520750711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}