{"id":"W2995773041","doi":"10.1186/s12896-019-0590-y","title":"Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography","year":2019,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Transgenic Plants and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Natural Science Foundation of China; York University; Ohio State University","keywords":"Nicotiana benthamiana; Green fluorescent protein; Recombinant DNA; Tobacco mosaic virus; Downstream processing; Biology; Chromatography; Biochemistry; Chemistry; Virus; Virology; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004123155,0.0006298078,0.0005664452,0.0003804144,0.0003304686,0.000343342,0.000482475,0.0004264755,0.0009974156],"category_scores_gemma":[0.0002651582,0.0002028299,0.0006071858,0.0005236786,0.0002516967,0.000333012,0.0003580055,0.000983255,0.001554921],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004793358,"about_ca_system_score_gemma":0.0005816876,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001849521,"about_ca_topic_score_gemma":0.002590419,"domain_scores_codex":[0.9996886,0.00003356933,0.00003258402,0.00006525912,0.0001301458,0.00004986722],"domain_scores_gemma":[0.9998461,0.00002587211,0.00003147998,0.00001988502,0.0000566377,0.00002009465],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001760602,0.0000172728,0.00009196433,0.00006414386,0.000005858502,0.00004766135,0.00001233317,0.00004690212,0.9973243,0.00007541218,0.0000855996,0.002210892],"study_design_scores_gemma":[0.00001838636,0.0001315246,0.002079452,0.00001290468,0.00003299674,0.0004881751,0.00003041151,0.002071679,0.985397,0.000182816,0.009533449,0.00002102694],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6305157,0.006135067,0.349025,0.001128965,0.000262206,0.001135636,0.003152026,0.001872767,0.006772662],"genre_scores_gemma":[0.6548362,0.006610025,0.3024108,0.0006301507,0.0001127271,0.0008906726,0.01545275,0.0004949606,0.01856171],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001849521,"threshold_uncertainty_score":0.003677547,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01460523784252782,"score_gpt":0.2548508933231383,"score_spread":0.2402456554806104,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}